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Growth performance, nucleic acids, leptin and adiponectin and their receptor gene expression were significantly affected by feeding different lipid supplementation in GIFT tilapia juveniles
Aquaculture Research ( IF 2 ) Pub Date : 2020-11-21 , DOI: 10.1111/are.14960
Zhaokun Ding 1 , Yongqiang Liu 2 , Xiaoli Liu 1 , Jinhua Han 1 , Yimin Zheng 1 , Weifeng Li 1, 3 , Youqing Xu 1
Affiliation  

The experiment was to investigate the effect of different dietary lipid levels on the growth performance, nucleic acids, leptin and adiponectin as well as their receptor gene expression in juvenile genetically improved farmed tilapia (GIFT, Oreochromis niloticus). Six groups of the juveniles with 40 days of age in triplicate were fed for 90 days using 6 iso‐nitrogen (34% dietary protein) diets. The 6 diets were control diet contained 0.35% of lipid, without lipid supplementation, and the other diets were added different doses of fish oil to make the different dietary lipid levels of 3.35%, 6.35%, 9.35%, 12.35% and 15.35% based on per kg of dried feed. The samples of whole fish and major tissues/organs were randomly taken at 0 and 90 days for determination, respectively. The main results were as follows: compared with the control diet, the diets with different lipid supplementation improved significantly (p < .05) the specific growth rate (SGR), crude fat, nucleic acids, adiponectin receptors 1 and 2 (AdipoRs 1 & 2) gene expression and serum leptin level in juvenile GIFT tilapia. However, the diets with different lipid supplementation reduced significantly (p < .05) the feed conversion ratio (FCR), hepatosomatic index (HSI), crude protein, leptin receptor (LepR) gene expression and serum adiponectin level. There was no significant effect (p > .05) on the survival rate (SR) in the juveniles fed the diets with different lipid supplementation. In conclusion, the diets with different lipid supplementation could affect significantly the SGR, FCR, HIS, nucleic acids, crude fat and protein, leptin and adiponectin levels and their receptor gene expression of the juveniles. The optimal dietary lipid level was 10.52% for SGR and 10.61% for FCR based on the second‐order polynomial regression analysis between dietary lipid levels against the SGR and FCR of the juveniles, respectively, in the experiment.

中文翻译:

GIFT罗非鱼幼鱼饲喂不同脂质后,其生长性能,核酸,瘦素和脂联素及其受体基因表达受到显着影响

该实验旨在研究不同饮食脂质水平对青少年基因改良养殖罗非鱼(GIFT,尼罗罗非鱼)的生长性能,核酸,瘦素和脂联素及其受体基因表达的影响。)。使用6种异氮(34%的日粮蛋白质)饮食对六组40天大的幼体进行了90天的喂养。这6种饮食是对照饮食,其中饮食中的脂质含量为0.35%,没有添加脂质,其他饮食中添加了不同剂量的鱼油,使不同饮食中的脂质含量分别为3.35%,6.35%,9.35%,12.35%和15.35%。每公斤干饲料。在第0天和第90天随机抽取全鱼和主要组织/器官的样本进行测定。主要结果如下:与对照饮食相比,不同脂质补充的饮食显着改善(p <.05)少年GIFT罗非鱼的特定生长率(SGR),粗脂肪,核酸,脂联素受体1和2(AdipoRs 1和2)基因表达和血清瘦素水平。但是,添加不同脂质的日粮显着降低(p  <.05)饲料转化率(FCR),肝体指数(HSI),粗蛋白,瘦蛋白受体(LepR)基因表达和血清脂联素水平。没有显着影响(p 饲喂不同脂质补充饲料的少年的存活率(SR)> .05)。总之,日粮中添加不同脂质可以显着影响青少年的SGR,FCR,HIS,核酸,粗脂肪和蛋白质,瘦素和脂联素水平及其受体基因表达。根据实验中饮食脂质水平相对于青少年的SGR和FCR的二阶多项式回归分析,SGR的最佳饮食脂质水平为10.52%,FCR的最佳饮食脂质水平为10.61%。
更新日期:2020-11-21
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