Dissecting the functional diversity of T cells is critical in elucidating mechanisms and in developing therapies for various diseases. Here, we designed a 31‐parameter (29‐color) panel to enable the characterization of T‐cell subsets and immunophenotyping of the human peripheral blood and lymph nodes using cell surface staining. In addition to adaptive T‐cell markers, TCR Vα24‐Jα18, TCR γδ, TCR Vɑ7.2, and CD161 were included to identify iNKT, γδ T, and MAIT cells, respectively, which are innate‐like T cells. C‐X‐C chemokine receptors (CXCR3, CXCR4, CXCR5, CXCR6) and C‐C motif chemokine receptors (CCR4, CCR6, CCR7) were included to enable the identification of Th cell subsets (Th1, Th2, Th17), Tfh cell subsets (Tfh1, Tfh2, Tfh17), and Th cells with specific homing capacities. Furthermore, in this panel, we also used markers for assessing cell differentiation (CD45RO, CD7), activation (CD57, CD95, HLA‐DR) and the expression of some cosignaling molecules (PD‐1, NKG2D, CD28). Particularly, CD69 and CD103 were included for the further analysis of tissue resident memory T (Trm) cells. This panel would enable the in‐depth immunophenotyping of human T‐cell subsets, and may be applied in the monitoring, prognosis, and mechanistic studies of various immune‐related diseases.

中文翻译：

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OMIP 071：使用表面标记对人 T 细胞亚群进行深度免疫表型分析的 31 参数流式细胞术面板

剖析 T 细胞的功能多样性对于阐明各种疾病的机制和开发治疗方法至关重要。在这里，我们设计了一个 31 参数（29 色）面板，以使用细胞表面染色对人类外周血和淋巴结进行 T 细胞亚群的表征和免疫表型分析。除了适应性 T 细胞标记物外，还包括 TCR Vα24-Jα18、TCR γδ、TCR Vɑ7.2 和 CD161，以分别识别 iNKT、γδ T 和 MAIT 细胞，它们是先天样 T 细胞。包括 C-X-C 趋化因子受体（CXCR3、CXCR4、CXCR5、CXCR6）和 C-C 基序趋化因子受体（CCR4、CCR6、CCR7）以识别 Th 细胞亚群（Th1、Th2、Th17）、Tfh 细胞子集（Tfh1、Tfh2、Tfh17）和具有特定归巢能力的 Th 细胞。此外，在这个面板中，我们还使用标记来评估细胞分化（CD45RO、CD7）、激活（CD57、CD95、HLA-DR）和一些共信号分子（PD-1、NKG2D、CD28）的表达。特别是，CD69 和 CD103 被包括用于组织驻留记忆 T (Trm) 细胞的进一步分析。该面板将能够对人类 T 细胞亚群进行深入的免疫表型分析，并可应用于各种免疫相关疾病的监测、预后和机制研究。