The aim of this study was to evaluate the effects of a natural soda water [Shi Han Quan (SHQ)] on hyperglycemia and plasma metabolic profiling and explore the mechanism using metabolomics techniques. Kun‐Ming mice weighing 26 ± 2 g were used for the hyperglycemia animal model with alloxan and divided into control, hyperglycemia (HG), and HG + SHQ soda water (SHQ) groups. The experiment lasted for 30 days. The plasma metabolomic profiling of mice was determined using ultrahigh‐pressure liquid chromatography‐quadrupole‐time of flight‐mass spectrometry. After the mice drank SHQ soda water, the levels of insulin and blood glucose were significantly lower in the SHQ group compared with the control group, and the level of insulin sensitivity [insulin sensitivity index (ISI)] was significantly higher in the SHQ group compared with the HG group. The mice in the different groups after SHQ intervention could be separated into distinct clusters, and nine major plasma metabolites with significant differences between groups were found closely associated with blood glucose and ISI. The metabolic pathway analysis of these metabolites involved abnormal fatty acid oxidation and phospholipid, acylcarnitine, and corticoid metabolism. The results suggested the metabolic changes and possible mechanism of SHQ improving the alloxan‐induced HG, and the findings provided insights into the prevention and control of HG and diabetes.

中文翻译：

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用超高压液相色谱-四极杆-飞行时间质谱法分析苏打水对高血糖小鼠的血浆代谢组学

这项研究的目的是评估天然苏打水[Shi Han Quan（SHQ）]对高血糖症和血浆代谢谱的影响，并使用代谢组学技术探讨其机制。体重为26±2 g的Kun-Ming小鼠用于具有四氧嘧啶的高血糖动物模型，分为对照组，高血糖（HG）和HG + SHQ苏打水（SHQ）组。实验持续了30天。使用超高压液相色谱-四极杆飞行时间质谱法测定小鼠的血浆代谢组学谱。小鼠饮用SHQ苏打水后，与对照组相比，SHQ组的胰岛素和血糖水平显着降低，与之相比，SHQ组的胰岛素敏感性[胰岛素敏感性指数（ISI）]水平显着升高。与HG集团合作。SHQ干预后，不同组中的小鼠可以分为不同的簇，并且发现组之间存在显着差异的9种主要血浆代谢物与血糖和ISI密切相关。这些代谢物的代谢途径分析涉及异常的脂肪酸氧化和磷脂，酰基肉碱和皮质激素代谢。结果提示SHQ的代谢变化和可能的机制改善了四氧嘧啶诱导的HG，这些发现为HG和糖尿病的预防和控制提供了见识。这些代谢物的代谢途径分析涉及异常的脂肪酸氧化和磷脂，酰基肉碱和皮质激素代谢。结果提示SHQ的代谢变化和可能的机制改善了四氧嘧啶诱导的HG，这些发现为HG和糖尿病的预防和控制提供了见识。这些代谢物的代谢途径分析涉及异常的脂肪酸氧化和磷脂，酰基肉碱和皮质激素代谢。结果提示SHQ的代谢变化和可能的机制改善了四氧嘧啶诱导的HG，这些发现为HG和糖尿病的预防和控制提供了见识。