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Ficolin-3 in rheumatic fever and rheumatic heart disease
Immunology Letters ( IF 4.4 ) Pub Date : 2020-11-21 , DOI: 10.1016/j.imlet.2020.11.006
Sandra Jeremias Catarino 1 , Fabiana Antunes Andrade 1 , Lorena Bavia 1 , Luiza Guilherme 2 , Iara Jose Messias-Reason 1
Affiliation  

Rheumatic fever (RF) and chronic rheumatic heart disease (RHD) are complications of oropharyngeal infection caused by Streptococcus pyogenes. Despite the importance of the complement system against infections and autoimmunity diseases, studies on the role of the lectin pathway in RF and RHD are scarce. Thus, our aim was to evaluate the association of ficolin-3 serum levels, FCN3 polymorphisms and haplotypes with the susceptibility to RF and RHD. We investigated 179 patients with a history of RF (126 RHD and 53 RF only) and 170 healthy blood donors as control group. Ficolin-3 serum concentrations were measured using enzyme-linked immunosorbent assay (ELISA). Three FCN3 single nucleotide polymorphisms (SNPs rs532781899, rs28362807 and rs4494157) were genotyped through the sequence-specific PCR method. Lower ficolin-3 serum levels were observed in RF patients when compared to controls (12.81 μg/mL vs. 18.14 μg/mL respectively, p < 0.0001, OR 1.22 [1.12–1.34]), and in RHD in comparison to RF only (RFo) (12.72 μg/mL vs. 14.29 μg/mL respectively, p = 0.016, OR 1.38 [1.06–1.80]). Low ficolin-3 levels (<10.7 μg/mL) were more common in patients (39.5 %, 30/76) than controls (20.6 %, 13/63, p = 0.018, OR = 2.51 [1.14–5.31]), and in RHD (44.4 %, 28/63) than RFo (15.4 %, 2/13, p = 0.007, OR = 3.08 [1.43–6.79]). On the other hand, FCN3 polymorphism/haplotypes were not associated with ficolin-3 serum levels or the disease. Low ficolin-3 levels might be associated with RF, being a potential marker of disease progression.



中文翻译:

Ficolin-3 在风湿热和风湿性心脏病中的作用

风湿热(RF)和慢性风湿性心脏病(RHD)是由化脓性链球菌引起的口咽部感染的并发症尽管补体系统对感染和自身免疫性疾病很重要,但关于凝集素途径在 RF 和 RHD 中作用的研究却很少。因此,我们的目的是评估ficolin -3 血清水平、FCN3多态性和单倍型与 RF 和 RHD 易感性的关联。我们调查了 179 名有 RF 病史的患者(126 名 RHD 和 53 名仅 RF)和 170 名健康献血者作为对照组。使用酶联免疫吸附试验 (ELISA) 测量 Ficolin-3 血清浓度。三个FCN3通过序列特异性 PCR 方法对单核苷酸多态性(SNP rs532781899、rs28362807 和 rs4494157)进行基因分型。与对照组相比,在 RF 患者中观察到较低的 ficolin-3 血清水平(分别为 12.81 μg/mL18.14 μg/mL,p < 0.0001,OR 1.22 [1.12-1.34]),而在 RHD 中,与仅 RF 相比( RFo)(分别为 12.72 μg/mL14.29 μg/mL,p = 0.016,或 1.38 [1.06–1.80])。低 ficolin-3 水平 (<10.7 μg/mL) 在患者 (39.5 %, 30/76) 中比对照组 (20.6 %, 13/63, p = 0.018, OR = 2.51 [1.14–5.31]) 更常见,并且RHD (44.4 %, 28/63) 比 RFo (15.4 %, 2/13, p = 0.007, OR = 3.08 [1.43–6.79])。另一方面,FCN3多态性/单倍型与 ficolin-3 血清水平或疾病无关。低 ficolin-3 水平可能与 RF 相关,是疾病进展的潜在标志物。

更新日期:2020-11-27
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