Abstract Novel composites based on poly(є-caprolactone)/polyethylene oxide loaded with hyaluronic acid(HA) and keratin(KR) were produced separately using emulsion and coaxial electrospinning methods. HA and KR were extracted from animal sources, characterized and loaded into coaxial fiber structures as bioactive agents, separately and together. Morphological, chemical, thermal, and mechanical characteristics of the fibers were investigated. According to the SEM results, diameters of smooth and beadless fibers fabricated via emulsion method were at nanoscale (sub-micron) while fibers of coaxial method were at micro scale. Benefitted electrospinning techniques demonstrated that hydrophobic and hydrophilic polymers can be advantageously combined. Core polymer specific FT-IR bands were not visible, their presence was proven with DSC analysis which confirms core-shell morphology of the fibers. In vitro studies exhibited spun mats did not have any cytotoxic effects and the HA and KR incorporated into the fiber structure synergistically increased cell viability and cell proliferation. This study demonstrated that the electrospun fibers containing HA and KR fabricated by both emulsion and coaxial methods can be efficient for wound healing applications.

中文翻译：

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用于伤口愈合应用的提取透明质酸和角蛋白纳米纤维的同轴和乳液静电纺丝

摘要 使用乳液和同轴静电纺丝方法分别制备了基于聚（є-己内酯）/聚环氧乙烷负载透明质酸（HA）和角蛋白（KR）的新型复合材料。HA 和 KR 是从动物来源中提取的，分别和一起作为生物活性剂表征并加载到同轴纤维结构中。研究了纤维的形态、化学、热和机械特性。根据扫描电镜结果，乳液法制备的光滑无珠纤维的直径为纳米级（亚微米级），而同轴法制备的纤维为微米级。受益的静电纺丝技术表明疏水性和亲水性聚合物可以有利地结合。核心聚合物特定的 FT-IR 波段不可见，DSC 分析证实了它们的存在，这证实了纤维的核壳形态。体外研究表明纺丝垫没有任何细胞毒性作用，并且结合到纤维结构中的 HA 和 KR 协同增加了细胞活力和细胞增殖。该研究表明，通过乳液和同轴方法制造的含有 HA 和 KR 的电纺纤维可有效用于伤口愈合应用。