The possibility of detecting the damaging effect of cadmium salts on red blood cells (RBC) membrane by atomic force microscopy and light microscopy was studied. White wistar rats RBC were incubated with cadmium chloride in concentrations of 1 μg/l, 10 μg/l, 100 μg/l, and 1000 μg/l for the research. A comparison of sample preparation methods proposed by other authors in previous studies is made. The optimal method that does not significantly affect the change in the morphological features of the cell is selected. The quantitative assessment of damaged and destroyed RBC depending on the concentration of cadmium was performed by optical microscopy. The study showed that CdCl₂ has a damaging effect on the RBC membrane, which leads to the formation of non-specific cell forms. A comparative assessment was made between the methods of optical microscopy and atomic force microscopy for the suitability of studying the morphological characteristics of abnormal forms of the RBC. It is shown that the method of atomic force microscopy allows registering morphological changes in the RBC that cannot be registered by optical microscopy. It is pointed that CdCl₂ has effect on destruction of the RBC and the formation of specific bulges on the RBC membrane. Influence of CdCl₂ on the RBC mechanical properties was studied using atomic force microscopy. The possibility of using atomic force microscopy in studies of morphology and mechanical properties of the RBC under toxicity effect of cadmium is shown.

研究了通过原子力显微镜和光学显微镜检测镉盐对红细胞（RBC）膜的破坏作用的可能性。将白色wistar大鼠RBC与氯化镉分别以1μg/ l，10μg/ l，100μg/ l和1000μg/ l的浓度孵育。对其他作者在先前研究中提出的样品制备方法进行了比较。选择不显着影响细胞形态特征变化的最佳方法。通过光学显微镜对受损和破坏的红细胞的定量评估取决于镉的浓度。研究表明，CdCl ₂对RBC膜具有破坏作用，导致形成非特异性细胞形式。在光学显微镜和原子力显微镜的方法之间进行了比较评估，以研究红细胞的异常形式的形态特征。结果表明，原子力显微镜法可以记录红细胞中的形态变化，而光学显微镜无法记录这种形态变化。指出CdCl ₂对RBC的破坏和在RBC膜上形成特定凸起的作用。CdCl _2的影响使用原子力显微镜研究了RBC的力学性能。显示了在镉的毒性作用下使用原子力显微镜研究RBC的形态和力学性能的可能性。