Abstract In global aquaculture industry, shrimp farming plays a key role and microalgae signify a vital part. The present study focussed to analyse the influence of dietary inclusion of Aurantiochytrium sp. MBTDCMFRIJMVL1, a heterotrophic marine protist as well as co-feeding with Artemia, on the growth and survival in black tiger shrimp (Penaeus monodon). The nutritive quality, with a total protein content of 54% and total fat content of 26% on dry matter basis along with good fatty acid profile and carotenoids makes Aurantiochytrium sp. a promising candidate for aquaculture nutrition. In the present study, three type of micro diets were designed (iso11-nitrogenous and isolipidic) with 50% crude protein and 8% crude fat viz., control diet (C), 1% inclusion of algae (T1) and 2% inclusion of algae (T2) and fed to the post larvae (PL) of P. monodon. There were six treatments in triplicate as follows; control (C), control accompanied by co-feeding with Artemia nauplii (C + A), 1% dietary inclusion of algae (T1), T1 with co-feeding (T1 + A), 2% dietary inclusion of algae (T2) and T2 along with co-feeding (T2 + A). 3600 numbers of PL with average length and weight 4.98 ± 0.13 mm and 1.51 ± 0.05 mg, respectively were randomly stocked in 18 rectangular glass tanks of 20 L water holding capacity with 200 PL in each tank and maintained at a salinity of 25‰. After the experimental period of 28 days, a better growth performance, survival rate, stress tolerance, nutritional quality and metabolic enzyme profile of the larvae were observed in ‘T2 + A' when compared to other treatments, while the lowest performance was observed in ‘C'. This study also concluded that, co-feeding with Artemia greatly enhanced the improvements brought by algal supplementation. Algal supplementation in the micro diet of P. monodon improved disease resistance against Vibrios. The fatty acid profile of PL28 were influenced due to algal supplementation. The levels of palmitic acid and docosahexaenoic acid (DHA) were higher and significantly different (P

中文翻译：

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微藻的膳食补充剂，Aurantiochytrium sp。与卤虫共同喂养可增强斑节对虾 (Fabricius, 1798) 幼虫后的生长、应激耐受性和存活率

摘要 在全球水产养殖业中，对虾养殖起着关键作用，而微藻则是至关重要的组成部分。本研究的重点是分析饮食中包含 Aurantiochytrium sp. 的影响。MBTDCMFRIJMVL1，一种异养海洋原生生物，并与卤虫共同喂养，对黑虎虾（斑节对虾）的生长和存活。营养品质，总蛋白质含量为 54%，总脂肪含量为 26%，以干物质为基础，以及良好的脂肪酸谱和类胡萝卜素，使 Aurantiochytrium sp。水产养殖营养的有希望的候选者。在本研究中，设计了三种类型的微量日粮（iso11-氮和等脂），粗蛋白为 50%，粗脂肪为 8%，即对照日粮 (C)、1% 的藻类 (T1) 和 2%藻类 (T2) 并喂给斑节对虾的后期幼虫 (PL)。一式三份地进行如下六次处理；对照（C），对照与卤虫无节幼体共同饲养（C + A），1％的藻类（T1），T1与共同饲养（T1 + A），2％的藻类（T2）和 T2 以及共同喂养 (T2 + A)。将3600只平均长度和重量分别为4.98±0.13mm和1.51±0.05mg的PL随机放养在18个20L容量的矩形玻璃罐中，每罐200PL，盐度保持在25‰。在28天的实验期后，与其他处理相比，在'T2 + A'中观察到幼虫的更好的生长性能、存活率、胁迫耐受性、营养品质和代谢酶谱，而在'中观察到最低性能。 C'。本研究还得出结论，与卤虫共同喂养大大增强了补充藻类带来的改善。在斑节对虾微量饮食中添加藻类提高了对弧菌的抗病性。PL28 的脂肪酸谱受到藻类补充的影响。棕榈酸和二十二碳六烯酸（DHA）水平较高且差异显着（P