Fertilization is one of the most important procedures in artificial reproduction and it directly affects the reproduction outcome. When there is optimization of fertilization, there can be a positive effect on subsequent reproductive processes and economic aspects of aquaculture. This study was conducted to determine time for which oocytes and sperm of barbel Barbus barbus retain fertilization capacity following placement in freshwater. Furthermore, the amount of ovarian fluid, excreted by fish during spawning with eggs (OFI; %) was determined, along with the chemical composition and effects on fertilization were determined. Gametes, ovarian fluid, and seminal plasma from barbel spawning specimens of the F₄ generation were used to conduct the study. Ovarian fluid accounted for 14%–68% of contents of the mass released at spawning and post-spawning composition differed depending on whether hormonal treatments were utilized for control of reproduction. There was an association (R² = 0.982; P = 0.000) between the pH of ovarian fluid and the barbel embryo survival rate. There was the greatest survival rate (>60 %) when the pH range of 7.9–8.4 and there was a lesser embryo viability when pH values were lesser or greater than values within this range (P < 0.05). The results from the study indicate that barbel eggs retain fertilization capacity longer (as long as 210 s) after activation by placement in fresh water than spermatozoa (about 30 s).

受精是人工繁殖中最重要的程序之一，它直接影响繁殖结果。当优化施肥时，可以对水产养殖的后续繁殖过程和经济方面产生积极影响。进行这项研究是为了确定 barbel Barbus barbus 的卵母细胞和精子在放入淡水后保留受精能力的时间。此外，还测定了鱼在产卵期间排出的卵巢液量 (OFI; %)，以及化学成分和对受精的影响。来自 F ₄触须产卵标本的配子、卵巢液和精浆一代被用来进行研究。卵巢液占产卵时释放的质量的 14%–68%，产卵后的成分根据是否使用激素处理来控制繁殖而有所不同。卵巢液的pH值与触须胚胎存活率之间存在关联（R ²  = 0.982；P = 0.000）。当 pH 值范围为 7.9-8.4 时，存活率最高（>60%），当 pH 值小于或大于该范围内的值时，胚胎存活率较低（P  < 0.05）。研究结果表明，与精子（约 30 秒）相比，在淡水中放置激活后，触须卵保持受精能力的时间更长（长达 210 秒）。