Particle bombardment, or biolistics, has emerged as an excellent alternative approach for plant genetic transformation which circumvents the limitations of Agrobacterium-mediated genetic transformation. The method has no biological constraints and can transform a wide range of plant species. Besides, it has been the most efficient way to achieve organelle transformation (for both chloroplasts and mitochondria) so far. Along with the recent advances in genome editing technologies, conventional gene delivery tools are now being repurposed to deliver targeted gene editing reagents into the plants. One of the key advantages is that the particle bombardment allows DNA-free gene editing of the genome. It enables the direct delivery of proteins, RNAs, and RNPs into plants. Owing to the versatility and wide-range applicability of the particle bombardment, it will likely remain one of the major genetic transformation methods in the future. This article provides an overview of the current status of particle bombardment technology and its applications in the field of plant research and biotechnology.

粒子轰击或生物弹已成为一种很好的植物遗传转化方法，它可以克服农杆菌的局限性介导的遗传转化。该方法无生物学限制，可以转化多种植物。此外，到目前为止，它是实现细胞器转化（叶绿体和线粒体均）的最有效方法。随着基因组编辑技术的最新发展，传统的基因传递工具现在正被重新用于将目标基因编辑试剂传递到植物中。关键优势之一是粒子轰击允许对基因组进行无DNA的基因编辑。它可以将蛋白质，RNA和RNP直接递送到植物中。由于粒子轰击的多功能性和广泛的适用性，它很可能在将来仍将是主要的遗传转化方法之一。