In this work, a novel and simple bone morphogenetic protein (BMP)-2 carrier is developed, which enables localized and controlled release of BMP-2 and facilitates bone regeneration. BMP-2 is localized in the gelatin methacrylate (GelMA) micropatterns on hydrophilic semi-permeable membrane (SNM), and its controlled release is regulated by the concentration of GelMA hydrogel and BMP-2. The controlled release of BMP-2 is verified using computational analysis and quantified using fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) diffusion model. The osteogenic differentiation of osteosarcoma MG-63 cells is manipulated by localized and controlled BMP-2 release. The calcium deposits are significantly higher and the actin skeletal networks are denser in MG-63 cells cultured in the BMP-2-immobilized GelMA micropattern than in the absence of BMP-2. The proposed BMP-2 carrier is expected to not only act as a barrier membrane that can prevent invasion of connective tissue during bone regeneration, but also as a carrier capable of localizing and controlling the release of BMP-2 due to GelMA micropatterning on SNM. This approach can be extensively applied to tissue engineering, including the localization and encapsulation of cells or drugs.

在这项工作中，开发了一种新颖，简单的骨形态发生蛋白（BMP）-2载体，该载体可实现BMP-2的局部和受控释放，并促进骨再生。BMP-2位于亲水性半透膜（SNM）上的甲基丙烯酸明胶（GelMA）微图案中，其受控释放受GelMA水凝胶和BMP-2浓度的调节。使用计算分析验证BMP-2的控制释放，并使用异硫氰酸荧光素-牛血清白蛋白（FITC-BSA）扩散模型进行定量。骨肉瘤MG-63细胞的成骨分化可通过局部和受控的BMP-2释放来控制。与没有BMP-2的情况相比，在BMP-2固定的GelMA微模式中培养的MG-63细胞中，钙的沉积量明显更高，肌动蛋白的骨骼网络更密集。预计提出的BMP-2载体不仅可以充当屏障膜，可以防止结缔组织在骨再生过程中侵入，而且还可以作为能够定位和控制BMP-2释放的载体，这是由于在SNM上进行了GelMA微图案化。这种方法可以广泛地应用于组织工程，包括细胞或药物的定位和封装。