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Antifungal effects of Curcuma longa L. essential oil against pathogenic strains isolated from indoor air
Aerobiologia ( IF 2 ) Pub Date : 2020-11-22 , DOI: 10.1007/s10453-020-09675-3
Erasmo Gámez-Espinosa , Matilde Anaya , Pedro Borges , Diana Marelys Bosch Crespo

Curcuma longa L. essential oil can be considered a natural product with strong antifungal activity due to the fungicidal and fungistatic effect. It can be used for the aeromycological control of premises where food is prepared. It is important to monitor air quality in food production facilities to achieve safe food. The objective of this work was to evaluate the antifungal activity of C. longa essential oil on potentially pathogenic and biodegradable strains isolated from the indoor of the food industry. A suspension of 106 spores/mL of the strains Aspergillus flavus, Penicillium brevicompactum and Cladosporium cladosporioides was prepared. Two natural biocides (C. longa essential oil and Cuban propolis) and two synthetic ones (antiseptic detergent and Tween 20) at different concentrations (0, 0.001, 0.1 and 1.0% v/v) were evaluated. The superficial growth of the mycelium (cm) and the germination inhibition (%) were the response variables. In addition, the minimum inhibitory concentration (MIC) of EO was determined by radial growth total inhibition (IRG) and partial sporulation inhibition (PIS). A. flavus colony diameter was decrease inversely proportional to the concentrations, and at 1% v/v it reduced more than three times its diameter, being selected as MIC since it caused IRG and PIS. Germination inhibition of the spores of the three strains was observed in relation directly proportional to the concentration of the four biocides. All spores with values similar to antiseptic detergent were inhibited by the essential oil at MIC (1%) between 41 and 55%

中文翻译:

姜黄精油对室内空气病原菌株的抗真菌作用

由于具有杀真菌和抑真菌作用,姜黄精油可以被认为是一种具有很强抗真菌活性的天然产物。它可用于制备食品的场所的空气真菌学控制。重要的是监测食品生产设施的空气质量以实现安全食品。这项工作的目的是评估 C. longa 精油对从食品工业室内分离的潜在致病和可生物降解菌株的抗真菌活性。制备了 106 个孢子/mL 的黄曲霉、短青霉和枝状枝孢菌菌株的悬浮液。评估了不同浓度(0、0.001、0.1 和 1.0% v/v)的两种天然杀菌剂(C. longa 精油和古巴蜂胶)和两种合成杀菌剂(防腐剂和吐温 20)。菌丝体的表面生长 (cm) 和萌发抑制 (%) 是响应变量。此外,EO 的最小抑制浓度 (MIC) 由径向生长总抑制 (IRG) 和部分孢子形成抑制 (PIS) 确定。A. flavus 菌落直径与浓度成反比减少,在 1% v/v 时,它减少了其直径的三倍以上,被选为 MIC,因为它会引起 IRG 和 PIS。观察到三种菌株孢子的萌发抑制与四种杀生物剂的浓度成正比。在 41% 到 55% 的 MIC (1%) 条件下,精油抑制了所有具有与防腐剂洗涤剂相似的值的孢子 EO的最小抑制浓度(MIC)由径向生长总抑制(IRG)和部分孢子形成抑制(PIS)确定。A. flavus 菌落直径与浓度成反比减少,在 1% v/v 时,它减少了其直径的三倍以上,被选为 MIC,因为它会引起 IRG 和 PIS。观察到三种菌株孢子的萌发抑制与四种杀生物剂的浓度成正比。在 41% 到 55% 的 MIC (1%) 条件下,精油抑制了所有具有与防腐剂洗涤剂相似的值的孢子 EO的最小抑制浓度(MIC)由径向生长总抑制(IRG)和部分孢子形成抑制(PIS)确定。A. flavus 菌落直径与浓度成反比减少,在 1% v/v 时,它减少了其直径的三倍以上,被选为 MIC,因为它会引起 IRG 和 PIS。观察到三种菌株孢子的萌发抑制与四种杀生物剂的浓度成正比。在 41% 到 55% 的 MIC (1%) 条件下,精油抑制了所有具有与防腐剂洗涤剂相似的值的孢子 并且在 1% v/v 时,它的直径减小了三倍以上,被选为 MIC,因为它会导致 IRG 和 PIS。观察到三种菌株孢子的萌发抑制与四种杀生物剂的浓度成正比。在 41% 到 55% 的 MIC (1%) 条件下,精油抑制了所有具有与防腐剂洗涤剂相似的值的孢子 并且在 1% v/v 时,它的直径减小了三倍以上,被选为 MIC,因为它会导致 IRG 和 PIS。观察到三种菌株孢子的萌发抑制与四种杀生物剂的浓度成正比。在 41% 到 55% 的 MIC (1%) 条件下,精油抑制了所有具有与防腐剂洗涤剂相似的值的孢子
更新日期:2020-11-22
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