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Tissue topography steers migrating Drosophila border cells
Science ( IF 56.9 ) Pub Date : 2020-11-19 , DOI: 10.1126/science.aaz4741
Wei Dai 1 , Xiaoran Guo 1 , Yuansheng Cao 2 , James A Mondo 1 , Joseph P Campanale 1 , Brandon J Montell 3 , Haley Burrous 1 , Sebastian Streichan 4 , Nir Gov 5 , Wouter-Jan Rappel 2 , Denise J Montell 1
Affiliation  

Cells and the path of least resistance For processes encompassing proper embryonic development, adult homeostasis, tumor cell dissemination, and immunity, certain cells must translocate from their site of origin. Migrating cells navigate physical features of their microenvironment; however, the in vivo importance of tissue topography for pathfinding is mostly unknown. Studying fruit flies, Dai et al. used border cells within the ovarian egg chamber to study path selection. Live imaging, genetics, mathematical modeling, and simulations showed that tissue microtopography provides an energetically favorable path of least resistance, whereas chemoattractants supply orthogonal guidance information and cell-cell adhesion contributes traction. The results provide insight into how cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path among many. Science, this issue p. 987 Cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path among many. Moving cells can sense and respond to physical features of the microenvironment; however, in vivo, the significance of tissue topography is mostly unknown. Here, we used Drosophila border cells, an established model for in vivo cell migration, to study how chemical and physical information influences path selection. Although chemical cues were thought to be sufficient, live imaging, genetics, modeling, and simulations show that microtopography is also important. Chemoattractants promote predominantly posterior movement, whereas tissue architecture presents orthogonal information, a path of least resistance concentrated near the center of the egg chamber. E-cadherin supplies a permissive haptotactic cue. Our results provide insight into how cells integrate and prioritize topographical, adhesive, and chemoattractant cues to choose one path among many.

中文翻译:

组织地形引导迁移果蝇边界细胞

细胞和阻力最小的路径 对于包括适当胚胎发育、成人体内平衡、肿瘤细胞传播和免疫在内的过程,某些细胞必须从它们的起源部位转移。迁移细胞在其微环境的物理特征中导航;然而,组织地形对寻路的体内重要性大多是未知的。研究果蝇,戴等人。使用卵巢卵室内的边界细胞来研究路径选择。实时成像、遗传学、数学建模和模拟表明,组织微地形提供了一条阻力最小的能量有利路径,而化学引诱物提供正交引导信息,细胞间粘附有助于牵引。结果提供了对细胞如何整合和优先考虑地形、粘合剂、和化学引诱线索从众多路径中选择一条。科学,这个问题 p。987 细胞整合并优先考虑地形、粘附和化学引诱因素,以从众多路径中选择一条。移动的细胞可以感知并响应微环境的物理特征;然而,在体内,组织形态学的意义大多是未知的。在这里,我们使用果蝇边界细胞(一种已建立的体内细胞迁移模型)来研究化学和物理信息如何影响路径选择。尽管人们认为化学线索就足够了,但实时成像、遗传学、建模和模拟表明微地形也很重要。化学引诱物主要促进向后运动,而组织结构呈现正交信息,阻力最小的路径集中在卵室中心附近。E-cadherin 提供了一个宽松的触觉提示。我们的结果提供了关于细胞如何整合和优先考虑地形、粘附和化学引诱线索以从众多路径中选择一条路径的见解。
更新日期:2020-11-19
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