Melanogenesis is a complex process that can lead to pigmentation defects. Various chemical skin-lightening products have been developed to treat pigmentation disorders. However, these chemical products can cause harmful adverse effects. Therefore, the development of safer, natural bleaching ingredients is a trend for sustainability. It has been reported that unsaturated fatty acids exhibit significant antimelanogenic effects. Sapindus mukorossi seed oils contain abundant unsaturated fatty acids; however, these have not yet been investigated for beneficial effects on skin tone evenness. In this study, we tested the possibility of using S. mukorossi oil for the treatment of hyperpigmentation in an in vitro model. Free fatty acid compositions and β-sitosterol were determined by gas chromatography-mass spectrometry and high-pressure liquid chromatography, respectively. The effect of S. mukorossi oil on melanoma B16F10 cell viability was detected using the 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyl-tetrazolium bromide assay. The inhibitive effects of fatty acids and β-sitosterol in S. mukorossi oil on α-melanocyte-stimulating hormone (MSH)-induced melanogenesis was evaluated by detecting melanin formation and tyrosinase activity. Our results showed that S. mukorossi oil produced no significant cytotoxicity in B16F10 cells at various concentrations compared with the control group. The enhancement of melanin formation induced by α-MSH was reduced by S. mukorossi oil. We also found that the primary fatty acid contributing to the antimelanogenesis effect was eicosenoic acid. These results suggest that S. mukorossi seed oil can effectively inhibit melanogenesis and has the potential for future development as a de-hyperpigmentation product within a waste utilization context.

黑色素生成是一个复杂的过程，可能导致色素沉着缺陷。已经开发出各种化学美白产品来治疗色素沉着症。但是，这些化学产品可能导致有害的不良影响。因此，开发更安全，天然的漂白成分是可持续发展的趋势。据报道，不饱和脂肪酸具有显着的促炭疽作用。Sapindus mukorossi种子油含有丰富的不饱和脂肪酸。然而，尚未研究这些对肤色均匀性的有益作用。在这项研究中，我们测试了使用S. mukorossi的可能性油用于在体外模型中治疗色素沉着过度。游离脂肪酸组成和β-谷甾醇分别通过气相色谱-质谱和高压液相色谱法测定。使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基-溴化四氮唑测定法检测了粘液链球菌油对黑素瘤B16F10细胞活力的影响。通过检测黑色素的形成和酪氨酸酶活性，评估了S. mukorossi油中脂肪酸和β-谷甾醇对α-黑素细胞刺激激素（MSH）诱导的黑素生成的抑制作用。我们的结果表明，S。mukorossi与对照组相比，不同浓度的豆油在B16F10细胞中均未产生明显的细胞毒性。S. mukorossi油减少了由α-MSH诱导的黑色素形成的增强。我们还发现，促红细胞生成作用的主要脂肪酸是二十碳烯酸。这些结果表明，S。mukorossi种子油可有效抑制黑色素生成，并有可能在废物利用的背景下作为脱色素沉着产品的未来发展。