The lipase production process by Thermomyces lanuginosus solid-state cultivation on hull-less pumpkin oil pomace was studied. A reduced quadratic model of lipase production was proposed based on the optimization of five independent variables followed by a model validation with 95% accuracy. Maximum lipase production was obtained after a process duration of 2 days with high volumetric and specific activity. The enzyme activity of 391 U g_db^–1 proves that the developed process is highly efficient. Partial purification of the crude enzymatic extracts was investigated, and a highly concentrated lipase liquid preparation with a volumetric activity of 422 U cm^–3 was achieved. The sustainability of the process was confirmed by a snapshot assessment using the green chemistry matrix of the E-factor and the process mass intensity, quoting on the mass efficiency. The new lipase process was in the range of the best white biotechnology processes, as per green chemistry metrics ranking.

中文翻译：

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产自嗜热霉菌的脂肪酶的可持续生产：工艺优化和酶表征

研究了无壳南瓜油渣固态发酵羊毛嗜热菌生产脂肪酶的过程。基于五个独立变量的优化，然后以95％的准确度进行模型验证，提出了一种简化的脂肪酶生产二次模型。经过2天的加工时间后，具有较高的体积和比活性，从而获得了最大的脂肪酶产量。391 U g _db ^–1的酶活性证明了所开发的过程是高效的。对粗酶提取物的部分纯化进行了研究，并制备了具有422 U cm ^–3体积活性的高浓度脂肪酶液体制剂已实现。通过使用E因子的绿色化学矩阵和过程质量强度，并参考质量效率，通过快照评估来确认过程的可持续性。根据绿色化学指标排名，新的脂肪酶工艺属于最佳的白色生物技术工艺。