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Tubular Silk Fibroin/Gelatin-Tyramine Hydrogel with Controllable Layer Structure and Its Potential Application for Tissue Engineering
ACS Biomaterials Science & Engineering ( IF 5.8 ) Pub Date : 2020-11-19 , DOI: 10.1021/acsbiomaterials.0c01183
Sheng Xu 1, 2, 3, 4, 5 , Qingtao Li 2, 3, 6 , Haotian Pan 1, 2, 3, 4, 5 , Qiyuan Dai 1, 2, 3, 4, 5 , Qi Feng 1, 2, 3, 4, 5 , Chenxi Yu 1, 2, 3, 4, 5 , Xiaohua Zhang 1, 2, 3, 4, 5 , Zhibin Liang 1, 2, 3, 4, 5 , Hua Dong 1, 2, 3, 4, 5 , Xiaodong Cao 1, 2, 3, 4, 5
Affiliation  

In recent years, biomimetic tubular scaffolds have been widely used to repair various human tissue defects, due to their hollow structure similar to the native tissues such as blood vessel, trachea, ureter, and bone marrow cavity. However, there are still many challenges in manufacturing a tubular hydrogel scaffold with suitable mechanical properties, specific microstructure, and good biocompatibility. In this study, we exploited an enzymatic cross-linking method using horseradish peroxidase (HRP) as an enzyme and hydrogen peroxide (H2O2) as a substrate, and combining with gelatin’s thermal sensitivity to produce an enzymatically cross-linked silk fibroin/gelatin-tyramine (E-SF/GT) tubular hydrogel. Through further treatment with methanol, we fabricated an EM-SF/GT tubular hydrogel with fine-wall architecture that consists of two different layers (inner and outer, dense and porous). Mechanical measurement showed that the compressive moduli values were up to 4.82 MPa and the tensile moduli values were up to 4.79 kPa under the static loading conditions. Also, degradation test showed that the hydrogel’s degradation time was prolonged. Finally, the bioactivity was tested by seeding mouse bone marrow mesenchymal stem cells (mBMSCs) in the lumen of a small-diameter (2 mm) EM-SF/GT tubular hydrogel. Cell morphology and immunofluorescence test indicated that mBMSCs differentiated into endothelial cells and lined the inner surface of the tubular hydrogel under induction. This work provided a feasible strategy for developing tubular hydrogels, which could be potentially used as scaffolds for hollow multilayer tissue engineering, such as blood vessels.

中文翻译:

具有可控层结构的管状丝素蛋白/明胶-酪胺水凝胶及其在组织工程中的潜在应用

近年来,仿生管状支架由于其类似于血管,气管,输尿管和骨髓腔等天然组织的中空结构而被广泛用于修复各种人体组织缺陷。然而,在制造具有合适的机械性能,特定的微结构和良好的生物相容性的管状水凝胶支架中仍然存在许多挑战。在这项研究中,我们利用辣根过氧化物酶(HRP)作为酶和过氧化氢(H 2 O 2)作为底物,并与明胶的热敏性相结合以生产酶促交联的丝素蛋白/明胶-酪胺(E-SF / GT)管状水凝胶。通过用甲醇进一步处理,我们制造了具有细壁结构的EM-SF / GT管状水凝胶,该结构由两个不同的层(内层和外层,致密层和多孔层)组成。力学测量表明,在静态载荷条件下,压缩模量最高为4.82 MPa,拉伸模量最高为4.79 kPa。另外,降解试验表明水凝胶的降解时间延长。最后,通过将小鼠骨髓间充质干细胞(mBMSC)植入小直径(2 mm)EM-SF / GT管状水凝胶的内腔中来测试生物活性。细胞形态学和免疫荧光测试表明,mBMSCs分化为内皮细胞,并在诱导下排列在管状水凝胶的内表面。这项工作为开发管状水凝胶提供了可行的策略,可将其用作中空多层组织工程(如血管)的支架。
更新日期:2020-12-14
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