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Male and female genotype and a genotype-by-genotype interaction mediate the effects of mating on cellular but not humoral immunity in female decorated crickets
Heredity ( IF 3.8 ) Pub Date : 2020-11-20 , DOI: 10.1038/s41437-020-00384-8 Kylie J Hampton 1 , Kristin R Duffield 1 , John Hunt 2, 3 , Scott K Sakaluk 1 , Ben M Sadd 1
Heredity ( IF 3.8 ) Pub Date : 2020-11-20 , DOI: 10.1038/s41437-020-00384-8 Kylie J Hampton 1 , Kristin R Duffield 1 , John Hunt 2, 3 , Scott K Sakaluk 1 , Ben M Sadd 1
Affiliation
Sexually antagonistic coevolution is predicted to lead to the divergence of male and female genotypes related to the effects of substances transferred by males at mating on female physiology. The outcome of mating should thus depend on the specific combination of mating genotypes. Although mating has been shown to influence female immunity in diverse insect taxa, a male-female genotype-by-genotype effect on female immunity post mating remains largely unexplored. Here, we investigate the effects of mating on female decorated cricket baseline immunity and the potential for a male-genotype-by-female-genotype interaction affecting this response. Females from three distinct genotypic backgrounds were left unmated or singly mated in a fully reciprocal design to males from the same three genotypic backgrounds. Hemocytes and hemocyte microaggregations were quantified for female cellular immunity, and phenoloxidase, involved in melanization, and antibacterial activity for humoral immunity. In this system, female cellular immunity was more reactive to mating, and mating effects were genotype-dependent. Specifically, for hemocytes, a genotype-by-mating status interaction mediated the effect of mating per se, and a significant male-female genotype-by-genotype interaction determined hemocyte depletion post mating. Microaggregations were influenced by the female's genotype or that of her mate. Female humoral immune measures were unaffected, indicating that the propensity for post-mating effects on females is dependent on the component of baseline immunity. The genotype-by-genotype effect on hemocytes supports a role of sexual conflict in post-mating immune suppression, suggesting divergence of male genotypes with respect to modification of female post-mating immunity, and divergence of female genotypes in resistance to these effects.
中文翻译:
雄性和雌性基因型和基因型间的相互作用介导交配对雌性蟋蟀细胞免疫而非体液免疫的影响
预计性对立的共同进化会导致雄性和雌性基因型的差异,这与雄性在交配时转移的物质对雌性生理的影响有关。因此,交配的结果应该取决于交配基因型的特定组合。尽管交配已被证明会影响多种昆虫类群中的雌性免疫力,但雄性 - 雌性基因型对交配后雌性免疫力的影响在很大程度上仍未得到探索。在这里,我们研究了交配对雌性装饰蟋蟀基线免疫的影响,以及影响这种反应的雄性-基因型-雌性-基因型相互作用的可能性。来自三个不同基因型背景的雌性未交配或以完全互惠的设计与来自相同三个基因型背景的雄性单独交配。血细胞和血细胞微聚集体被量化用于女性细胞免疫,酚氧化酶参与黑色素化和体液免疫的抗菌活性。在这个系统中,雌性细胞免疫对交配反应更强烈,并且交配效应是基因型依赖性的。具体而言,对于血细胞,交配状态下的基因型相互作用介导了交配本身的影响,并且显着的雄性-雌性基因型-基因型相互作用决定了交配后的血细胞消耗。微聚集受到雌性基因型或其配偶基因型的影响。雌性体液免疫措施不受影响,这表明交配后对雌性的影响的倾向取决于基线免疫的组成部分。
更新日期:2020-11-20
中文翻译:
雄性和雌性基因型和基因型间的相互作用介导交配对雌性蟋蟀细胞免疫而非体液免疫的影响
预计性对立的共同进化会导致雄性和雌性基因型的差异,这与雄性在交配时转移的物质对雌性生理的影响有关。因此,交配的结果应该取决于交配基因型的特定组合。尽管交配已被证明会影响多种昆虫类群中的雌性免疫力,但雄性 - 雌性基因型对交配后雌性免疫力的影响在很大程度上仍未得到探索。在这里,我们研究了交配对雌性装饰蟋蟀基线免疫的影响,以及影响这种反应的雄性-基因型-雌性-基因型相互作用的可能性。来自三个不同基因型背景的雌性未交配或以完全互惠的设计与来自相同三个基因型背景的雄性单独交配。血细胞和血细胞微聚集体被量化用于女性细胞免疫,酚氧化酶参与黑色素化和体液免疫的抗菌活性。在这个系统中,雌性细胞免疫对交配反应更强烈,并且交配效应是基因型依赖性的。具体而言,对于血细胞,交配状态下的基因型相互作用介导了交配本身的影响,并且显着的雄性-雌性基因型-基因型相互作用决定了交配后的血细胞消耗。微聚集受到雌性基因型或其配偶基因型的影响。雌性体液免疫措施不受影响,这表明交配后对雌性的影响的倾向取决于基线免疫的组成部分。