Cyclic di-nucleotides are important secondary signaling molecules in bacteria that regulate a wide range of processes. In this study, we found that Caenorhabditis elegans can detect and are attracted to multiple signal molecules produced by Vibrio cholerae, specifically the 3′,5′-cyclic diguanylate (c-di-GMP), even though this bacterium kills the host at a high rate. C-di-GMP is sensed through C. elegans olfactory AWC neurons, which then evokes a series of signal transduction pathways that lead to reduced activity of two key stress response transcription factors, SKN-1 and HSF-1, and weakened innate immunity. Taken together, our study elucidates the role of c-di-GMP in interkingdom communication. For C. elegans, bacterial c-di-GMP may serve as a cue that they can use to detect food. On the other hand, preexposure to low concentrations of c-di-GMP may impair their immune response, which could facilitate bacterial invasion and survival.

环二核苷酸是细菌中重要的次级信号分子，可调节多种过程。在这项研究中，我们发现秀丽隐杆线虫可以检测并被霍乱弧菌产生的多个信号分子吸引，特别是3'，5'-环状双鸟苷酸（c-di-GMP），即使该细菌在宿主处杀死了宿主。高几率。C-di-GMP通过秀丽隐杆线虫嗅觉AWC神经元感知，然后唤起一系列信号转导途径，导致两个关键应激反应转录因子SKN-1和HSF-1的活性降低，并削弱先天免疫力。综上所述，我们的研究阐明了c-di-GMP在神经交际中的作用。对于线虫，细菌c-di-GMP可能会提示他们可以用来检测食物。另一方面，预先暴露于低浓度的c-di-GMP可能会削弱其免疫反应，从而促进细菌的侵袭和存活。