当前位置: X-MOL 学术Mol. Reprod. Dev. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Reduced growth capacity of preimplantation mouse embryos in chronic unpredictable stress model
Molecular Reproduction and Development ( IF 2.5 ) Pub Date : 2020-11-20 , DOI: 10.1002/mrd.23439
Xiaoli Zhao 1 , Ruihong Ma 1 , Xiaoyu Zhang 1 , Rui Cheng 1 , Nan Jiang 1 , Mengjia Guo 1 , Beilei Rong 1 , Yan Liu 1 , Mingli Chen 1 , Weihua Feng 1 , Tian Xia 1
Affiliation  

Psychological stress can affect female reproduction by deteriorating oocyte quality, but the molecular mechanism is unclear. In this study, we used the chronic unpredictable stress model to study the effect of psychological stress on mouse oocyte competence during preimplantation stage, and RNA sequencing in single oocytes to analyze differential gene expression at the transcription level. Stress changed the serum levels of glucocorticoids and reduced oocyte developmental potential, depending on the strength of the stress. Strong stress (two stressors per day) reduced the fertilization rate and induced significant apoptosis in blastocysts. Moderate stress (one stressor per day) reduced the cleavage rate and blastocyst formation rate. Weak stress (one stressor every 2 days) did not have any significant negative effect on the fertilization, cleavage, and blastocyst formation. Hatching rate was not affected by stress, but stress retarded the development of the expanded blastocysts and inhibited the embryo development at early stages. Transcriptome analysis revealed that stress disturbed the expression of cell cycle regulators and apoptotic genes. The hub genes identified through protein‐protein interaction analysis include Msln, Ceacam12, Psg16, Psg17, and Psg23, which are all carcinoembryonic or related genes involved in cell adhesion, proliferation, and migration. Thus, stress was inhibitory on fertilization and early embryo development in mice.

中文翻译:

慢性不可预测应激模型中植入前小鼠胚胎的生长能力降低

心理压力可通过降低卵母细胞质量来影响女性生殖,但分子机制尚不清楚。在本研究中,我们使用慢性不可预测压力模型来研究心理压力对胚胎植入前阶段小鼠卵母细胞能力的影响,并使用单个卵母细胞的 RNA 测序来分析转录水平的差异基因表达。根据压力的强度,压力会改变糖皮质激素的血清水平并降低卵母细胞的发育潜力。强烈的压力(每天两个压力源)降低了受精率并诱导了囊胚的显着细胞凋亡。中等压力(每天一个压力源)会降低卵裂率和囊胚形成率。弱压力(每 2 天一个压力源)对受精、卵裂、和囊胚形成。孵化率不受应激的影响,但应激阻碍了膨胀囊胚的发育并抑制了早期胚胎的发育。转录组分析表明,压力干扰了细胞周期调节因子和凋亡基因的表达。通过蛋白质-蛋白质相互作用分析鉴定的枢纽基因包括MslnCeacam12Psg16Psg17Psg23都是与细胞粘附、增殖和迁移有关的癌胚或相关基因。因此,压力对小鼠的受精和早期胚胎发育具有抑制作用。
更新日期:2021-01-28
down
wechat
bug