The present study investigated Rhoeo discolor (L. Her.) Hance for its ability to accumulate Pb, which is of relevance to phytoremediation applications. Based on this analysis, plants were found to accumulate greater than 10 mg/g (0.1%) of dry weight Pb in the shoots, which classifies the plant a Pb hyperaccumulator. Further, changes in the leaf proteome profiles in response to Pb stress were investigated. Wild‐type plants were subjected to a high concentration of Pb(NO₃)₂, and the levels of Pb that accumulated in different plant tissues were determined using atomic absorption spectrophotometry. Using 2D‐difference gel electrophoresis, 181 protein spots were detected to be differentially abundant in response to Pb stress and selected spots exhibiting the strongest differential abundance suggested an impairment of the photosynthetic apparatus of the plant under Pb stress. Subsequently, a more extensive, proteome wide analysis utilizing label‐free quantitation further identified a predominant decrease in protein levels and a significant effect on the nuclear proteome, as well as photosynthesis, carbon fixation and metabolism, providing insight into the Pb tolerance of this system in a potential phytoremediation context.

中文翻译：

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铅超积累物，Rhoeo变色（L. Her。）Hance叶片的差异蛋白质组分析

本研究调查了Rhoeo变色（L. Her。）Hance积累Pb的能力，这与植物修复应用有关。根据此分析，发现植物在枝条中积累的干重Pb大于10 mg / g（0.1％），这将植物归类为Pb超富集者。此外，研究了响应于Pb胁迫的叶片蛋白质组图谱的变化。野生型植物受到高浓度的Pb（NO ₃）_2的作用，并使用原子吸收分光光度法测定了在不同植物组织中积累的铅水平。使用2D差异凝胶电泳，检测到181个蛋白质斑点响应Pb胁迫而差异丰富，并且选定的斑点表现出最强的差异丰度，这表明在Pb胁迫下植物的光合装置受到了损害。随后，利用无标记定量的蛋白质组学更广泛的分析进一步确定了蛋白质水平的显着下降以及对核蛋白质组以及光合作用，碳固定和新陈代谢的显着影响，从而提供了对该系统对Pb耐受性的洞察力在潜在的植物修复环境中。