The recent advances in liquid‐phase transmission electron microscopy represent tremendous potential in many different fields and exciting new opportunities. However, achieving both high‐resolution imaging and operando capabilities remain a significant challenge. This work suggests a novel in situ imaging platform of liquid‐flowing graphene chip TEM (LFGC‐TEM) equipped with graphene viewing windows and a liquid exchange system. The LFGCs are robust under high‐pressure gradients and rapid liquid circulation in ranges covering the experimental conditions accessible with conventional thick SiN_x chips. LFGC‐TEM provides atomic resolution for colloidal nanoparticles and molecular‐level information limits for unstained wet biomolecules and cells that are comparable to the resolutions achievable with solid‐phase and cryogenic TEM, respectively. This imaging platform can provide an opportunity for live imaging of biological phenomena that is not yet achieved using any current methods.

中文翻译：

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基于液体流动石墨烯芯片的高分辨率电子显微镜

液相透射电子显微镜的最新进展代表了许多不同领域的巨大潜力和令人兴奋的新机遇。然而，实现高分辨率成像和操作能力仍然是一个重大挑战。这项工作提出了一种新型的液体流动石墨烯芯片 TEM (LFGC-TEM) 原位成像平台，该平台配备了石墨烯观察窗和液体交换系统。LFGC 在高压梯度和快速液体循环下是稳健的，其范围涵盖了常规厚 SiN _x可达到的实验条件筹码。LFGC-TEM 为胶体纳米粒子提供了原子分辨率，为未染色的湿生物分子和细胞提供了分子水平的信息限制，分别与固相和低温 TEM 可达到的分辨率相当。该成像平台可以为使用任何当前方法尚未实现的生物现象进行实时成像提供机会。