Dual-signal ratiometric platforms (DSRPs) have an intrinsic built-in correction to interference from background signals, and show improved accuracy and reliability for target detection. This review summarizes the construction principles of DSRPs with different electric signal transduction modes, and highlights biosensing applications at live cell and small animal levels. Firstly, we discuss construction principles of DSRPs, involving DSRPs with one or two inherent signal sources, target-induced signal changes, signal transduction modes and particular principles. Then, we highlight biosensing applications of DSRPs at live cell and small animal levels, involving various biological targets and samples, sensing mechanisms, biosensing of cell extracts and inherent targets in live cells, biosensing and determination of live cells, biosensing of targets at live small animal level. Finally, challenges and perspectives for construction principles and electrochemical biosensing applications of DSRPs are illustrated. This review promotes the development of current chemo/bio-sensing techniques, intelligent biosensors and electronic devices.

双信号比率测量平台（DSRP）具有内在的内置校正功能，可以对背景信号进行干扰，并且可以提高目标检测的准确性和可靠性。这篇综述总结了具有不同电信号传导模式的DSRP的构建原理，并重点介绍了在活细胞和小型动物水平上的生物传感应用。首先，我们讨论了DSRP的构造原理，包括具有一个或两个固有信号源，目标引起的信号变化，信号传导模式和特定原理的DSRP。然后，我们重点介绍了DSRP在活细胞和小型动物水平上的生物传感应用，涉及各种生物靶标和样品，传感机制，活细胞中细胞提取物和固有靶​​标的生物传感，活细胞的生物传感和测定，在活体小动物水平上对目标进行生物传感。最后，说明了DSRPs的构建原理和电化学生物传感应用的挑战和前景。这篇评论促进了当前的化学/生物传感技术，智能生物传感器和电子设备的发展。