Introduction

In-body tissue architecture (iBTA) technology, based on cell-free tissue engineering, can produces collagenous tissues for implantation by subcutaneous embedding a designed mold. The aim of this study was to evaluate the biocompatibility of iBTA-induced “Biosheet®” collagenous sheets, as scaffold materials for bladder reconstruction.

Methods

Canine Biosheet® implants were prepared by embedding molds into subcutaneous pouches in beagles for 8 weeks. A part of canine bladder wall was excised (2 × 2 cm) and repaired by patching the same sized autologous Biosheet®. The Biosheet® implants were harvested 4 weeks (n = 1) and 12 weeks (n = 3) after the implantation and evaluated histologically.

Results

No disruption of the patched Biosheet® implants or urinary leakage into the peritoneal cavity was observed during the entire observation periods. There were no signs of chronic inflammation or Biosheet® rejection. The urine-contacting surface of luminal surface of the Biosheet® was covered with a multicellular layer of urothelium cells 4 weeks after implantation. α-SMA-positive muscle cells were observed at the margin of the Biosheet® implants at 12 weeks after the implantation. In addition, in the center of the Biosheet® implants, the formation of microvessels stained as α-SMA-positive was observed.

Conclusion

Biosheet® implants have biocompatibility as a scaffold for bladder reconstruction, indicating that they may be applicable for full-thickness bladder wall substitution. Further studies are required for definitive evaluation as a scaffold for bladder reconstruction.

中文翻译：

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使用体内组织结构诱导的自体胶原结缔组织膜“Biosheet®”进行膀胱重建：一项试点研究

介绍

体内组织架构（iBTA）技术基于无细胞组织工程，可以通过皮下嵌入设计的模具来产生用于植入的胶原组织。本研究的目的是评估 iBTA 诱导的“Biosheet®”胶原片作为膀胱重建支架材料的生物相容性。

方法

Canine Biosheet® 植入物是通过将模具嵌入比格犬的皮下袋中 8 周来制备的。切除犬膀胱壁的一部分（2 × 2 cm）并通过修补相同尺寸的自体 Biosheet® 进行修复。Biosheet® 种植体在植入后 4 周（n = 1）和 12 周（n = 3）收获并进行组织学评估。

结果

在整个观察期间，没有观察到打补丁的 Biosheet® 植入物的破坏或尿液渗漏到腹膜腔中。没有慢性炎症或 Biosheet® 排斥的迹象。植入后 4 周，Biosheet® 管腔表面的尿液接触面被尿路上皮细胞的多细胞层覆盖。植入后 12 周，在 Biosheet® 植入物的边缘观察到 α-SMA 阳性肌肉细胞。此外，在 Biosheet® 植入物的中心，观察到染色为 α-SMA 阳性的微血管的形成。

结论

Biosheet®植入物作为膀胱重建支架具有生物相容性，这表明它们可能适用于全层膀胱壁替代。作为膀胱重建支架的明确评估还需要进一步的研究。