Cnaphalocrocis medinalis granulovirus (CnmeGV) is a potential microbial agent against the rice leaffolder. Innate immunity is essential for insects to survive pathogenic infection. Therefore, to clarify the immune response of Cnaphalocrocis medinalis to the viral colonization, the gene expression profile of C. medinalis infected with CnmeGV was constructed by RNA-seq. A total of 8,503 differentially expressed genes (DEGs) were found including 5,304 up-regulated and 3,199 down-regulated unigenes. Gene enrichment analysis indicated that these DEGs were mainly linked to protein synthesis and metabolic process as well as ribosome and virus-infection pathways. Specifically, a significantly up-regulated PiggyBac-like transposon gene was identified suggested that the enhancement of transposon activity is related to host immunity. Further, the DEGs encoding oxidative stress related genes were identified and validated by RT-qPCR. Overall, 9 antioxidant enzyme genes and 4 antioxidant protein genes were up-regulated, and the extensive glutathione S-transferase genes were down-regulated. Our results provide a basis for understanding the molecular mechanisms of baculovirus action and oxidative stress response in C. medinalis and other insects.

稻纵卷叶螟颗粒体（CnmeGV）是针对稻纵卷叶螟一个潜在的微生物剂。先天免疫对于昆虫在病原体感染中生存至关重要。因此，为了阐明的免疫应答稻纵卷叶螟于病毒定植，的基因表达谱稻纵卷叶螟感染CnmeGV物通过RNA-SEQ构成。总共发现了8,503个差异表达基因（DEG），包括5,304个上调和3,199个下调的单基因。基因富集分析表明，这些DEGs主要与蛋白质合成和代谢过程以及核糖体和病毒感染途径有关。具体来说，像PiggyBac一样明显上调转座子基因的鉴定表明转座子活性的增强与宿主免疫力有关。此外，通过RT-qPCR鉴定并验证了编码氧化应激相关基因的DEG。总体而言，上调了9个抗氧化酶基因和4个抗氧化蛋白基因，而广泛的谷胱甘肽S-转移酶基因被下调。我们的研究结果提供用于理解杆状病毒作用和氧化应激反应的分子机制的基础稻纵卷叶螟和其他昆虫。