Background

Non-small-cell lung cancer (NSCLC) is the most frequent type of lung cancer and more than 90 % of mortality is due to metastasis-related deaths. Flavonoids are considered nutraceuticals due to the variety of pharmacological properties. In this paper, we studied the effects of baicalin, silibinin, apigenin, luteolin, and its oxidovanadium(IV) cation complexes on the viability, adhesion to fibronectin, invasion, and migration on human lung cancer cell line A549. In addition, in order to complete the study of the interaction of VOflavonoids and bovine serum albumin (BSA), the binding ability of silibinin and VOsil to the protein was evaluated.

Method

To establish the non-cytotoxic concentration range of the tested compounds, the cancer cell viability was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. Cell migration and invasion assays were performed using Boyden chambers and adhesion assay using MTT method. The interaction of compounds with BSA were investigated in physiological buffer (pH = 7.4) by fluorescence spectroscopy.

Results

All complexes inhibited the metastatic cascade steps to a greater extent than their respective ligands. Likewise, based on binding constant values (K_b) for BSA-silibinin and BSA-VOsil, we can suggest that both compounds can interact with the protein.

Conclusion

Although all the complexes suppressed cell adhesion, invasion and migration, VOlut can be considered as a good candidate to continue the trials because it presented encouraging results as a potential antitumor and antimetastatic agent, and can be transported by BSA.

中文翻译：

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VO类黄酮复合物对A549细胞系的抗转移作用

背景

非小细胞肺癌 (NSCLC) 是最常见的肺癌类型，超过 90% 的死亡率是由于转移相关的死亡。由于多种药理特性，类黄酮被认为是营养品。在本文中，我们研究了黄芩苷、水飞蓟宾、芹菜素、木犀草素及其氧化钒 (IV) 阳离子复合物对人肺癌细胞系 A549 的活力、对纤连蛋白的粘附、侵袭和迁移的影响。此外，为了完成VO类黄酮与牛血清白蛋白（BSA）相互作用的研究，评估了水飞蓟宾和VOsil对蛋白质的结合能力。

方法

为了确定测试化合物的非细胞毒性浓度范围，使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-四唑溴化物 (MTT) 测定法评估癌细胞活力。使用 Boyden 室进行细胞迁移和侵袭测定，使用 MTT 方法进行粘附测定。通过荧光光谱法在生理缓冲液 (pH = 7.4) 中研究了化合物与 BSA 的相互作用。

结果

所有复合物都比它们各自的配体在更大程度上抑制转移级联步骤。同样，基于BSA-水飞蓟宾和 BSA-VOsil 的结合常数值 (K _b )，我们可以建议这两种化合物都可以与蛋白质相互作用。

结论

尽管所有复合物都抑制了细胞粘附、侵袭和迁移，但 VOlut 可以被认为是继续试验的良好候选者，因为它作为潜在的抗肿瘤和抗转移剂呈现出令人鼓舞的结果，并且可以通过 BSA 转运。