The Blue Bottle Experiment involves a reversible redox process of decolorizing methylene blue (MB) by reaction with glucose (Glc) at basic pH. The direction of the reaction is affected by the concentration of dissolved O₂. In this work, the reactions that took place in the presence and absence of nanoparticles of smectites (a group of layered silicates) were compared. The method of absorption spectroscopy with a diode-array detector was chosen for studying the reaction. It was possible to measure the full spectrum in a fraction of a second, and thus several hundred spectra were obtained for each experiment. Spectral matrices were analyzed using the chemometric method of multivariate curve resolution (MCR). Analysis of the results revealed not only the formation of known reaction products (a leuco-form of MB, the oxidation products of Glc) but also some other processes related to the changes of the reactants in a basic environment, slow dye decomposition, glucose isomerization, and the formation of MB molecular aggregates. The injection of colloidal smectite particles into the reaction system effectively stopped the redox reaction of MB with Glc, which may be important for dye stabilization in hybrid materials with smectites. This property was not related to the type of smectite, which instead affected the molecular aggregation occurring in the heterogeneous systems.

的蓝瓶实验涉及通过在碱性pH与葡萄糖（GLC）反应脱色亚甲蓝（MB）的一个可逆的氧化还原过程。反应的方向受溶解的O ₂浓度的影响。在这项工作中，比较了在蒙脱石（一组层状硅酸盐）存在和不存在的情况下发生的反应。选择了带有二极管阵列检测器的吸收光谱法来研究反应。可以在不到一秒的时间内测量整个光谱，因此每个实验都获得了数百个光谱。使用多元曲线分辨率（MCR）的化学计量学方法分析光谱矩阵。结果分析表明，不仅形成了已知的反应产物（MB的无色形式，Glc的氧化产物），而且还发现了一些与碱性环境中反应物变化，染料缓慢分解，葡萄糖异构化有关的其他过程。 ，并形成MB分子聚集体。将胶体蒙脱石颗粒注入反应体系可有效停止MB与Glc的氧化还原反应，这对于与蒙脱石的杂化材料中的染料稳定化可能很重要。该特性与蒙脱石的类型无关，而蒙脱石的类型影响了异质体系中发生的分子聚集。