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Age-related changes in the immunomodulatory effects of human dental pulp derived mesenchymal stem cells on the CD4+ T cell subsets
Cytokine ( IF 3.8 ) Pub Date : 2021-02-01 , DOI: 10.1016/j.cyto.2020.155367
Rabia Bilge Özgül Özdemir 1 , Alper Tunga Özdemir 2 , Cengiz Kırmaz 3 , Ayla Eker Sarıboyacı 4 , Erdal Karaöz 5 , Gülay Erman 6 , H Seda Vatansever 7 , Nihal Mete Gökmen 8
Affiliation  

Mesenchymal stem cells (MSCs) are powerful immunomodulatory cells. The effects of the aging on these abilities of MSCs have not been adequately clarified. In this study, alterations in immunomodulatory abilities of MSCs caused by aging were investigated. For this, dental pulp (DP) MSCs and peripheral blood mononuclear cells (PBMCs) of elderly and young donors were co-cultured age-matched and cross. We detected that the effects of DP-MSCs on Th1 and Th2 cells and their specific cytokines IFN-γ and IL-4 are not affected by aging. However, we observed that young and elderly DP-MSCs have different effects on Th17 and Treg cells. Th17 frequencies of young and elderly PBMCs were significantly increased only by young DP-MSCs, in contrast, Treg frequencies were significantly increased by elderly DP-MSCs. IL-6, IL-17a and HGF levels of both young and elderly PBMCs showed a significant increase only by young DP-MSCs, but TGF-β levels were significantly increased only by elderly DP-MSCs. The oral cavity is home to a rich microflora. The interactions of dental tissues with this microflora can lead them to acquire different epigenetic modifications. Aging can affect the microflora composition of the oral cavity and change this process in different directions. According to our findings, DP-MSCs are effective cells in the regulation of CD4+ T cells, and their effects on Th1 and Th2 cells were not affected by aging. However, pleiotropic molecules IL-6 and HGF expressions, which are important in dental and bone tissue regeneration, decreased significantly in elderly DP-MSCs. This situation may have indirectly made a difference in the modulation effects of young and elderly DP-MSCs on the Th17 and Treg cells.

中文翻译:

人牙髓间充质干细胞对 CD4+ T 细胞亚群免疫调节作用的年龄相关变化

间充质干细胞 (MSCs) 是强大的免疫调节细胞。衰老对MSCs这些能力的影响尚未得到充分阐明。在这项研究中,研究了由衰老引起的 MSCs 免疫调节能力的改变。为此,将老年和年轻供体的牙髓(DP)MSC和外周血单核细胞(PBMC)进行年龄匹配和交叉共培养。我们检测到 DP-MSCs 对 Th1 和 Th2 细胞及其特异性细胞因子 IFN-γ 和 IL-4 的影响不受衰老的影响。然而,我们观察到年轻和老年 DP-MSCs 对 Th17 和 Treg 细胞有不同的影响。只有年轻的 DP-MSCs 显着增加了年轻和老年 PBMC 的 Th17 频率,相比之下,老年 DP-MSCs 显着增加了 Treg 频率。IL-6,年轻和老年 PBMC 的 IL-17a 和 HGF 水平仅在年轻 DP-MSCs 中显着增加,但 TGF-β 水平仅在老年 DP-MSCs 中显着增加。口腔是丰富微生物群落的家园。牙齿组织与这种微生物区系的相互作用可以导致它们获得不同的表观遗传修饰。衰老会影响口腔的微生物群落组成,并在不同方向改变这一过程。根据我们的研究结果,DP-MSCs是调节CD4+ T细胞的有效细胞,它们对Th1和Th2细胞的作用不受衰老的影响。然而,对牙齿和骨组织再生很重要的多效性分子 IL-6 和 HGF 表达在老年 DP-MSCs 中显着降低。
更新日期:2021-02-01
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