Originally developed in bacterial and fungal models, Adaptive Laboratory Evolution (ALE) experimentally selects for targeted phenotypes by allowing cells containing beneficial random genetic mutations to replicate more quickly in the presence of environmental stress. When applied to microalgae, this technique has been used to increase growth rate, stress tolerance, substrate utilization, and product yield. This review discusses ALE experimental design parameters, and summarizes key results and lessons learned from recent microalgal ALE studies. By documenting the current state of the field, we provide insight on the ways microalgal ALE might be used to develop commercially viable biotechnology strains.

最初是在细菌和真菌模型中开发的，通过允许包含有益的随机遗传突变的细胞在环境压力下能够更快地复制，自适应实验室进化（ALE）通过实验选择了目标表型。当应用于微藻时，该技术已被用于提高生长速率，胁迫耐受性，基质利用率和产品产量。这篇评论讨论了ALE实验设计参数，并总结了从最近的微藻ALE研究中获得的主要结果和经验教训。通过记录该领域的当前状况，我们提供了有关微藻ALE用于开发商业上可行的生物技术菌株的方法的见解。