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Development and characterisation of cytocompatible polyester substrates with tunable mechanical properties and degradation rate
Acta Biomaterialia ( IF 9.7 ) Pub Date : 2020-11-20 , DOI: 10.1016/j.actbio.2020.11.026
Sofia Ribeiro , Ana M. Carvalho , Emanuel M. Fernandes , Manuela E. Gomes , Rui L. Reis , Yves Bayon , Dimitrios I. Zeugolis

Although it has been repeatedly indicated the importance to develop implantable devices and cell culture substrates with tissue-specific rigidity, current commercially available products, in particular cell culture substrates, have rigidity values well above most tissues in the body. Herein, six resorbable polyester films were fabricated using compression moulding with a thermal presser into films with tailored stiffness by appropriately selecting the ratio of their building up monomers (e.g. lactide, glycolide, trimethylene carbonate, dioxanone, ε-caprolactone). Typical NMR and FTIR spectra were obtained, suggesting that the fabrication process did not have a negative effect on the conformation of the polymers. Surface roughness analysis revealed no apparent differences between the films as a function of polymer composition. Subject to polymer composition, polymeric films were obtained with glass transition temperatures from -52 °C to 61 °C; contact angles in water from 81 ° to 94 °; storage modulus from 108 MPa to 2,756 MPa and loss modulus from 8 MPa to 507 MPa (both in wet state, at 1 Hz frequency and at 37 °C); ultimate tensile strength from 8 MPa to 62 MPa, toughness from 23 MJ/m3 to 287 MJ/m3, strain at break from 3 % to 278 %, macro-scale Young's modulus from 110 MPa to 2,184 MPa (all in wet state); and nano-scale Young's modulus from 6 kPa to 15,019 kPa (in wet state). With respect to in vitro degradation in phosphate buffered saline at 37 °C, some polymeric films [e.g. poly(glycolide-lactide) 30 / 70] started degrading from day 7 (shortest timepoint assessed), whilst others [e.g. poly(glycolide-co-ε-caprolactone) 10 / 90] were more resilient to degradation up to day 21 (longest timepoint assessed). In vitro biological analysis using human dermal fibroblasts and a human monocyte cell line (THP-1) showed the potential of the polymeric films to support cell growth and controlled immune response. Evidently, the selected polymers exhibited properties suitable for a range of clinical indications.



中文翻译:

具有可调节机械性能和降解速率的细胞相容性聚酯底物的开发和表征

尽管已经反复表明开发具有组织特异性刚度的可植入装置和细胞培养基质的重要性,但是当前可商购的产品,特别是细胞培养基质,其刚度值远高于体内大多数组织。在此,通过适当地选择其积聚单体(例如丙交酯,乙交酯,碳酸三亚甲基酯,二恶烷酮,ε-己内酯)的比例,使用热压机通过压缩成型将六种可吸收性聚酯膜制成具有定制刚度的膜。获得了典型的NMR和FTIR光谱,表明制备过程对聚合物的构象没有负面影响。表面粗糙度分析表明,膜之间没有明显的差异,这是聚合物组成的函数。取决于聚合物组成,获得的聚合物膜的玻璃化转变温度为-52°C至61°C;水中接触角从81°到94°; 储能模量为108 MPa至2756 MPa,损耗模量为8 MPa至507 MPa(均为湿态,频率为1 Hz,温度为37°C); 极限抗拉强度从8 MPa到62 MPa,韧性从23 MJ / m3至287 MJ / m 3,断裂应变从3%至278%,宏观杨氏模量从110 MPa至2,184 MPa(均处于湿态);纳米级杨氏模量为6 kPa至15019 kPa(湿态)。关于在37°C下磷酸盐缓冲盐水中的体外降解,某些聚合物薄膜[例如聚(乙交酯-丙交酯)30/70]从第7天开始降解(评估的最短时间点),而其他[例如聚(乙交酯-丙交酯) -ε-己内酯(10/90)直到第21天(评估的最长时间点)对降解的抵抗力更强。体外使用人类真皮成纤维细胞和人类单核细胞系(THP-1)进行的生物学分析显示,聚合物膜支持细胞生长和控制免疫反应的潜力。显然,所选择的聚合物表现出适合于一系列临床适应症的性质。

更新日期:2021-01-28
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