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Plasma lipidomic profiles after a low and high glycemic load dietary pattern in a randomized controlled crossover feeding study
Metabolomics ( IF 3.6 ) Pub Date : 2020-11-20 , DOI: 10.1007/s11306-020-01746-3
Sepideh Dibay Moghadam 1, 2 , Sandi L Navarro 1 , Ali Shojaie 3 , Timothy W Randolph 1 , Lisa F Bettcher 4 , Cynthia B Le 4 , Meredith A Hullar 1 , Mario Kratz 1 , Marian L Neuhouser 1 , Paul D Lampe 1 , Daniel Raftery 1, 4 , Johanna W Lampe 1
Affiliation  

Background

Dietary patterns low in glycemic load are associated with reduced risk of cardiometabolic diseases. Improvements in serum lipid concentrations may play a role in these observed associations.

Objective

We investigated how dietary patterns differing in glycemic load affect clinical lipid panel measures and plasma lipidomics profiles.

Methods

In a crossover, controlled feeding study, 80 healthy participants (n = 40 men, n = 40 women), 18–45 y were randomized to receive low-glycemic load (LGL) or high glycemic load (HGL) diets for 28 days each with at least a 28-day washout period between controlled diets. Fasting plasma samples were collected at baseline and end of each diet period. Lipids on a clinical panel including total-, VLDL-, LDL-, and HDL-cholesterol and triglycerides were measured using an auto-analyzer. Lipidomics analysis using mass-spectrometry provided the concentrations of 863 species. Linear mixed models and lipid ontology enrichment analysis were implemented.

Results

Lipids from the clinical panel were not significantly different between diets. Univariate analysis showed that 67 species on the lipidomics panel, predominantly in the triacylglycerol class, were higher after the LGL diet compared to the HGL (FDR < 0.05). Three species with FA 17:0 were lower after LGL diet with enrichment analysis (FDR < 0.05).

Conclusion

In the context of controlled eucaloric diets with similar macronutrient distribution, these results suggest that there are relative shifts in lipid species, but the overall pool does not change. Further studies are needed to better understand in which compartment the different lipid species are transported in blood, and how these shifts are related to health outcomes.

This trial was registered at clinicaltrials.gov as NCT00622661.



中文翻译:

随机对照交叉喂养研究中低和高血糖负荷饮食模式后的血浆脂质组学特征

背景

低血糖负荷的饮食模式与降低心脏代谢疾病的风险有关。血清脂质浓度的改善可能在这些观察到的关联中起作用。

客观的

我们研究了不同血糖负荷的饮食模式如何影响临床脂质组测量和血浆脂质组学特征。

方法

在一项交叉对照喂养研究中,80 名 18-45 岁的健康参与者(n = 40 名男性,n = 40 名女性)随机接受低血糖负荷 (LGL) 或高血糖负荷 (HGL) 饮食,每次 28 天控制饮食之间至少有 28 天的清除期。在基线和每个饮食期结束时收集空腹血浆样品。使用自动分析仪测量临床面板上的脂质,包括总胆固醇、极低密度脂蛋白胆固醇、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇和甘油三酯。使用质谱法进行的脂质组学分析提供了 863 种物质的浓度。实施了线性混合模型和脂质本体富集分析。

结果

来自临床小组的脂质在饮食之间没有显着差异。单变量分析表明,与 HGL 相比,LGL 饮食后脂质组学面板上的 67 个物种(主要是三酰甘油类)更高(FDR < 0.05)。在经过富集分析的 LGL 饮食后,FA 17:0 的三个物种较低(FDR < 0.05)。

结论

在具有类似宏量营养素分布的受控低热量饮食的背景下,这些结果表明脂质种类存在相对变化,但总体池没有变化。需要进一步研究以更好地了解不同脂质种类在血液中的哪个隔室运输,以及这些变化如何与健康结果相关。

该试验在clinicaltrials.gov 注册为NCT00622661。

更新日期:2020-11-21
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