In this study, we aimed to isolate, identify and characterize lactic acid bacteria (LAB) from the intestine of juvenile seabass (Lates calcarifer) as a new potential probiotic. Four strains of LABs were isolated from the intestines of ten healthy seabass juveniles. In the in vitro screening process using spot lawn assay, one isolate labeled as LAB3 showed inhibitory activity against Vibrio harveyi (ATCC 35,084). Strain LAB3 was determined to belong to the gram positive bacteria group with cocci shape and was identified as Enterococcus hirae using 16S rDNA analysis. This bacterium was able to grow at pH ranging from pH 2 to 10 with the best growth at pH 7. This strain was also able to grow at 0-4% NaCl after 24 h incubation and grew best at 1.5% NaCl. Enterococcus hirae strain LAB3 of the present study is worthy to be further characterized as a potential probiotic for use in seabass culture.

中文翻译：

---

从鲈鱼 (Lates Calcarifer) 的肠道中分离出的平肠球菌的特性作为一种新的潜在益生菌对抗病原性弧菌

在这项研究中，我们旨在从幼年鲈鱼（Lates calcarifer）的肠道中分离、鉴定和表征乳酸菌（LAB）作为一种新的潜在益生菌。从 10 只健康鲈鱼幼鱼的肠道中分离出 4 株 LAB。在使用斑点草坪试验的体外筛选过程中，一种标记为 LAB3 的分离株显示出对哈维弧菌 (ATCC 35,084) 的抑制活性。菌株LAB3被确定属于具有球菌形状的革兰氏阳性菌群，并且使用16S rDNA分析被鉴定为平肠球菌。这种细菌能够在 pH 2 到 10 的 pH 范围内生长，在 pH 7 时生长最好。该菌株在孵育 24 小时后也能够在 0-4% NaCl 下生长，并且在 1.5% NaCl 下生长最好。