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Literature-Based Phenotype Survey and In Silico Genotype Investigation of Antibiotic Resistance in the Genus Bifidobacterium
Current Microbiology ( IF 2.6 ) Pub Date : 2020-10-14 , DOI: 10.1007/s00284-020-02230-w Linyan Cao , Huahai Chen , Qinghao Wang , Baiyuan Li , Yunfei Hu , Changhui Zhao , Yongfei Hu , Yeshi Yin
Current Microbiology ( IF 2.6 ) Pub Date : 2020-10-14 , DOI: 10.1007/s00284-020-02230-w Linyan Cao , Huahai Chen , Qinghao Wang , Baiyuan Li , Yunfei Hu , Changhui Zhao , Yongfei Hu , Yeshi Yin
Bifidobacteria are typical commensals inhabiting the human intestine and are beneficial to the host because of their probiotic properties. One of the risks concerning probiotics is the potential of introducing antibiotic resistance genes (ARGs) to the host gut pathogens. This study was aimed to depict the general antibiotic resistance characteristics of the genus Bifidobacterium by combining the reported phenotype dataset and in silico genotype prediction. Bifidobacteria were mostly reported to be sensitive to beta-lactams, glycopeptides, chloramphenicol, and rifampicin, but resistant to aminoglycosides, polypeptides, quinolones, and mupirocin. Generally, the resistance phenotypes to erythromycin, tetracycline, fusidic acid, metronidazole, clindamycin, and trimethoprim were variable. Besides cmX and tetQ, characterized in bifidobacterial resident plasmids, 3520 putative ARGs were identified from 831 bifidobacterial genomes through BLASTP search. The identified ARGs matched thirty-eight reference ARGs, four of which seemed to be mutant housekeeping genes. The two high-abundant ARGs, tetW and ermX, were found to have different distribution traits. The predicted ARGs reasonably explained most of the corresponding resistant phenotypes in the published literature.
中文翻译:
双歧杆菌属抗生素耐药性的基于文献的表型调查和计算机基因型调查
双歧杆菌是居住在人体肠道中的典型共生菌,由于其益生菌特性,对宿主有益。益生菌的风险之一是将抗生素抗性基因 (ARG) 引入宿主肠道病原体的可能性。本研究旨在通过结合报告的表型数据集和计算机基因型预测来描述双歧杆菌属的一般抗生素抗性特征。据报道,双歧杆菌对β-内酰胺类、糖肽类、氯霉素和利福平敏感,但对氨基糖苷类、多肽、喹诺酮类和莫匹罗星耐药。一般来说,对红霉素、四环素、夫西地酸、甲硝唑、克林霉素和甲氧苄啶的耐药表型是可变的。除了 cmX 和 tetQ,以双歧杆菌驻留质粒为特征,通过 BLASTP 搜索从 831 个双歧杆菌基因组中鉴定了 3520 个假定的 ARG。鉴定出的 ARG 与 38 个参考 ARG 相匹配,其中四个似乎是突变的看家基因。发现两个高丰度的 ARG,tetW 和 ermX,具有不同的分布特征。预测的 ARG 合理地解释了已发表文献中大多数相应的抗性表型。
更新日期:2020-10-14
中文翻译:
双歧杆菌属抗生素耐药性的基于文献的表型调查和计算机基因型调查
双歧杆菌是居住在人体肠道中的典型共生菌,由于其益生菌特性,对宿主有益。益生菌的风险之一是将抗生素抗性基因 (ARG) 引入宿主肠道病原体的可能性。本研究旨在通过结合报告的表型数据集和计算机基因型预测来描述双歧杆菌属的一般抗生素抗性特征。据报道,双歧杆菌对β-内酰胺类、糖肽类、氯霉素和利福平敏感,但对氨基糖苷类、多肽、喹诺酮类和莫匹罗星耐药。一般来说,对红霉素、四环素、夫西地酸、甲硝唑、克林霉素和甲氧苄啶的耐药表型是可变的。除了 cmX 和 tetQ,以双歧杆菌驻留质粒为特征,通过 BLASTP 搜索从 831 个双歧杆菌基因组中鉴定了 3520 个假定的 ARG。鉴定出的 ARG 与 38 个参考 ARG 相匹配,其中四个似乎是突变的看家基因。发现两个高丰度的 ARG,tetW 和 ermX,具有不同的分布特征。预测的 ARG 合理地解释了已发表文献中大多数相应的抗性表型。