This study reports the properties of immobilized MAS1-H108A lipase from marine Streptomyces sp. strain W007 on XAD1180 resin and its application in the synthesis of n-3 polyunsaturated fatty acids (PUFA)-rich triacylglycerols (TAG) for the first time. It was found that the optimal temperature and pH for both immobilized MAS1-H108A lipase and free lipase MAS1-H108A were 70 °C and 7.0, respectively. However, immobilized MAS1-H108A lipase exhibited higher thermostability when compared with free lipase MAS1-H108A. It was also interesting that both immobilized MAS1-H108A lipase and free lipase MAS1-H108A showed no regiospecificity in the hydrolysis of triolein. Subsequently, immobilized MAS1-H108A lipase and free lipase MAS1-H108A were employed to catalyze glycerolysis of n-3 PUFA-rich ethyl esters (EE) and esterification of n-3 PUFA with glycerol under vacuum in the solvent-free system. The results showed that n-3 PUFA-rich TAG were synthesized efficiently by non-regiospecific immobilized MAS1-H108A lipase and TAG contents separately reached 92.07% and 76.13% during the esterification and glycerolysis reactions, which were significantly higher than those (71.82% and 39.62%, respectively) obtained by free lipase MAS1-H108A. Besides, TAG exhibited similar n-3 PUFA composition to the substrate. These findings indicated that non-regiospecific immobilized MAS1-H108A lipase is a promising and efficient biocatalyst for the industrial synthesis of n-3 PUFA-rich TAG.

本研究报告了来自海洋链霉菌属的固定化 MAS1-H108A 脂肪酶的特性。菌株 W007 在 XAD1180 树脂上的首次应用及其在合成富含 n-3 多不饱和脂肪酸 (PUFA) 的三酰甘油 (TAG) 中的应用。发现固定化 MAS1-H108A 脂肪酶和游离脂肪酶 MAS1-H108A 的最适温度和 pH 值分别为 70 °C 和 7.0。然而，与游离脂肪酶 MAS1-H108A 相比，固定化 MAS1-H108A 脂肪酶表现出更高的热稳定性。同样有趣的是，固定化的 MAS1-H108A 脂肪酶和游离脂肪酶 MAS1-H108A 在甘油三油酸酯的水解中都没有表现出区域特异性。随后，固定化的 MAS1-H108A 脂肪酶和游离脂肪酶 MAS1-H108A 用于催化富含 n-3 PUFA 的乙酯 (EE) 的甘油解以及在无溶剂系统中真空下 n-3 PUFA 与甘油的酯化。结果表明，非区域特异性固定化 MAS1-H108A 脂肪酶可高效合成富含 n-3 PUFA 的 TAG，酯化和甘油解反应过程中 TAG 含量分别达到 92.07% 和 76.13%，明显高于（71.82% 和 76.13%）。 39.62%，分别）由游离脂肪酶 MAS1-H108A 获得。此外，TAG 表现出与基材相似的 n-3 PUFA 组成。这些发现表明，非区域特异性固定化 MAS1-H108A 脂肪酶是一种有前途且有效的生物催化剂，可用于工业合成富含 n-3 PUFA 的 TAG。分别）通过游离脂肪酶 MAS1-H108A 获得。此外，TAG 表现出与基材相似的 n-3 PUFA 组成。这些发现表明，非区域特异性固定化 MAS1-H108A 脂肪酶是一种有前途且有效的生物催化剂，可用于工业合成富含 n-3 PUFA 的 TAG。分别）通过游离脂肪酶 MAS1-H108A 获得。此外，TAG 表现出与基材相似的 n-3 PUFA 组成。这些发现表明，非区域特异性固定化 MAS1-H108A 脂肪酶是一种有前途且有效的生物催化剂，可用于工业合成富含 n-3 PUFA 的 TAG。