Living systems provide a promising approach to chemical synthesis, having been optimized by evolution to convert renewable carbon sources such as glucose to an enormous range of small molecules. However, a large number of synthetic structures can still be difficult to obtain solely from cells, such as unsubstituted hydrocarbons. In this work, we demonstrate the use of a hybrid cellular-heterogeneous catalytic strategy to produce olefins from glucose, using a selective hydrolase to generate an activated intermediate that is readily deoxygenated. Using a new family of iterative thiolase enzymes, we have genetically engineered a microbial strain that produces 4.3 (0.4) g L-1 of fatty acid from glucose with 86% captured as 3-hydroxyoctanoic and 3-hydroxydecanoic acids. This 3-hydroxy substituent serves as a leaving group enabling heterogeneous tandem decarboxylation-dehydration routes to olefinic products on Lewis acidic catalysts without the additional redox input required for enzymatic or chemical deoxygenation of simple fatty acids.

中文翻译：

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从葡萄糖生产烯烃的混合细胞和异构催化剂策略。

生命系统为化学合成提供了一种有前途的方法，并且已经通过进化进行了优化，可以将可再生碳源（例如葡萄糖）转化为各种小分子。但是，仍然很难仅从细胞中获得大量合成结构，例如未取代的烃。在这项工作中，我们证明了使用杂化的细胞异质催化策略从葡萄糖生产烯烃，使用选择性水解酶生成易于脱氧的活化中间体。使用一个新的迭代硫解酶家族，我们对一种微生物菌株进行了基因改造，该菌株可从葡萄糖中产生4.3（0.4）g L-1脂肪酸，其中86％被捕获为3-羟基辛酸和3-羟基癸酸。