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Superoxide anion generation response to wound in Arabidopsis hypocotyl cutting
Plant Signaling & Behavior ( IF 2.9 ) Pub Date : 2020-11-19 , DOI: 10.1080/15592324.2020.1848086 Xufang Ren 1 , Mengfang Wang 1 , Yongshun Wang 1 , Aixia Huang 1
Plant Signaling & Behavior ( IF 2.9 ) Pub Date : 2020-11-19 , DOI: 10.1080/15592324.2020.1848086 Xufang Ren 1 , Mengfang Wang 1 , Yongshun Wang 1 , Aixia Huang 1
Affiliation
Cutting is a frequently used model to study the process of adventitious root formation, and excision of cuttings leads to rapid wound response signaling. We recently showed that as a wound signal, reactive oxygen species (ROS, mainly hydrogen peroxide) participate in adventitious root induction of hypocotyl cuttings through regulation of auxin biosynthesis and transport. Here, superoxide anion (O2 -•), an early type of ROS, exhibited rapid burst at the cutting site immediately in response to wounding in Arabidopsis hypocotyl cuttings. Diphenylene iodonium chloride (DPI, inhibitor of NADPH oxidase) overwhelmingly suppressed O2 -• propagation through the hypocotyl. Compared to wild type, O2 -• burst only occur in cut base, and upward transduction were inhibited completely in NADPH oxidase mutant AtRbohD. These results indicate O2 -• generation and propagation in response to wound and via NADPH oxidase in adventitious root induction of hypocotyl cuttings.
中文翻译:
拟南芥下胚轴切割伤口的超氧阴离子生成反应
切割是研究不定根形成过程的常用模型,切割切割可产生快速的伤口反应信号。我们最近表明,作为伤口信号,活性氧(ROS,主要是过氧化氢)通过调节生长素生物合成和运输参与下胚轴插条的不定根诱导。在这里,超氧阴离子 (O2 -•),一种早期类型的 ROS,在切割部位立即表现出快速爆发,以响应拟南芥下胚轴插条中的伤口。二亚苯基氯化碘(DPI,NADPH 氧化酶抑制剂)压倒性地抑制了 O2 -• 通过下胚轴的传播。与野生型相比,O2-•爆裂仅发生在切割基部,NADPH氧化酶突变体AtRbohD中向上转导完全受到抑制。
更新日期:2020-11-19
中文翻译:
拟南芥下胚轴切割伤口的超氧阴离子生成反应
切割是研究不定根形成过程的常用模型,切割切割可产生快速的伤口反应信号。我们最近表明,作为伤口信号,活性氧(ROS,主要是过氧化氢)通过调节生长素生物合成和运输参与下胚轴插条的不定根诱导。在这里,超氧阴离子 (O2 -•),一种早期类型的 ROS,在切割部位立即表现出快速爆发,以响应拟南芥下胚轴插条中的伤口。二亚苯基氯化碘(DPI,NADPH 氧化酶抑制剂)压倒性地抑制了 O2 -• 通过下胚轴的传播。与野生型相比,O2-•爆裂仅发生在切割基部,NADPH氧化酶突变体AtRbohD中向上转导完全受到抑制。