Recycled cooking oil (RCO) is widely used in many small restaurants. However, the health risk posed by long-term consumption of RCO is unclear. In this study, C57 mice were treated with RCO for 34 weeks. Organ coefficients of the liver, stomach, and kidney were found to be decreased. H&E staining revealed overt lesions in the pancreas, liver, kidney, esophagus, duodenum, and ileum of RCO-treated mice. Immunohistochemistry showed significant DNA damage in the duodenum and ileum and apoptosis in the lungs of the RCO-treated mice. Immunoblotting showed elevated levels of γ-H2AX, Bcl-2/Bax, TNFα, cleaved Caspase-3 and poly ADP-ribose polymerase (PARP). Increased levels of lactate dehydrogenase (LDH) and decreased levels of succinate dehydrogenase (SDH) were also detected. These findings suggest that long-term consumption of RCO produces various toxicities in mice with important implications for humans. DNA damage followed by mitochondria-associated apoptosis, and necrosis is likely to contribute to the toxicities.

中文翻译：

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长期食用再生食用油会导致细胞死亡和组织损伤

再生食用油 (RCO) 广泛用于许多小餐馆。然而，长期食用 RCO 带来的健康风险尚不清楚。在这项研究中，C57 小鼠接受了 RCO 治疗 34 周。发现肝脏、胃和肾脏的器官系数降低。H&E 染色显示 RCO 治疗小鼠的胰腺、肝脏、肾脏、食道、十二指肠和回肠有明显病变。免疫组织化学显示，在 RCO 治疗的小鼠的十二指肠和回肠中存在显着的 DNA 损伤以及在肺中发生细胞凋亡。免疫印迹显示 γ-H2AX、Bcl-2/Bax、TNFα、裂解的 Caspase-3 和聚 ADP-核糖聚合酶 (PARP) 水平升高。还检测到乳酸脱氢酶 (LDH) 水平升高和琥珀酸脱氢酶 (SDH) 水平降低。这些发现表明，长期食用 RCO 会对小鼠产生各种毒性，对人类具有重要意义。DNA 损伤，随后是线粒体相关的细胞凋亡，坏死可能导致毒性。