IMPORTIN‐α3/MOS6 (MODIFIER OF SNC1, 6) is one of nine importin‐α isoforms in Arabidopsis that recruit nuclear localization signal‐containing cargo proteins to the nuclear import machinery. IMP‐α3/MOS6 is required genetically for full autoimmunity of the nucleotide‐binding leucine‐rich repeat immune receptor mutant snc1 (suppressor of npr1‐1, constitutive 1) and MOS6 also contributes to basal disease resistance. Here, we investigated the contribution of the other importin‐α genes to both types of immune responses, and we analyzed potential interactions of all importin‐α isoforms with SNC1. By using reverse‐genetic analyses in Arabidopsis and protein−protein interaction assays in Nicotiana benthamiana, we provide evidence that among the nine α‐importins in Arabidopsis, IMP‐α3/MOS6 is the main nuclear transport receptor of SNC1, and that IMP‐α3/MOS6 is required selectively for autoimmunity of snc1 and basal resistance to mildly virulent Pseudomonas syringae in Arabidopsis.

中文翻译：

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NLR蛋白SNC1介导的拟南芥importin-α核转运受体家族在自身免疫中的功能要求

IMPORTIN-α3/ MOS6（SNC1，6的修饰子）是拟南芥中将含有核定位信号的货物蛋白募集到核输入机器中的九种importin-α同工型之一。遗传上需要IMP-α3 / MOS6，以实现核苷酸结合的富含亮氨酸的重复免疫受体突变体snc1（npr1-1的抑制剂，组成型1）的完全自身免疫，而MOS6也有助于基础疾病的抵抗。在这里，我们研究了其他importin- α基因对两种类型的免疫应答的贡献，并分析了所有importin-α同工型与SNC1的潜在相互作用。通过在拟南芥中使用反向基因分析和本氏烟草的蛋白-蛋白相互作用分析，我们提供了证据，证明拟南芥中的九种α-重要蛋白中，IMP-α3/ MOS6是SNC1的主要核转运受体，并且IMP-α3 / MOS6是选择性的自发性免疫。SNC1和轻度有毒基础抗性丁香假单胞菌在拟南芥中。