Vibriosis caused by luminous Vibrio species is one of the biggest challenges to shrimp industry in Bangladesh. This study aimed to characterize whole microbial communities from Vibrio‐infected black tiger shrimp (Penaeus monodon) using 16S rRNA‐based amplicon sequencing. A total of 36 disease‐free and infected shrimp were collected from six different hatcheries in Bagerhat, Bangladesh. A final pool of 12 samples (n = 6) was created by homogenization of the hepatopancreas samples from three shrimps collected from each hatchery for the same group. The amplicon sequencing data revealed significant (p < .05) decrease of alpha diversity measurements and subsequent effects (p < .05) on the hepatopancreas microbiota in the infected group, compared to control shrimp. Proteobateria and Aeromonas were the most dominant bacteria at phylum and genus level in both groups and identified as core microbiota in the community. Two bacterial groups at phyla level and eight at genus level were found associated with the alteration of hepatopancreas microbial communities and associated gene functions in vibriosis‐infected shrimp, revealed by differential abundance and KEGG pathway analysis. The overwhelming abundance of Citroibacter, Shewanella and Candidatus lineages in vibriosis‐infected shrimp needs further investigations.

中文翻译：

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扩增子测序显示弧菌病感染的黑虎虾（斑节对虾）中弧菌丰度和相关基因功能显着增加

发光弧菌引起的弧菌病是孟加拉国虾类产业面临的最大挑战之一。这项研究旨在使用基于16S rRNA的扩增子测序来表征弧菌感染的黑虎虾（斑节对虾）的整个微生物群落。从孟加拉国Bagerhat的6个不同的孵化场中收集了总共36株无病和受感染的虾。 通过均质化来自同一孵化场的每个孵化场的三只虾的肝胰脏样本，最终创建了12个样本（n = 6）的最终库。扩增子测序数据显示 α多样性测量值显着降低（p <.05）和后续影响（p 与对照虾相比，感染组的肝胰腺微生物群上的<.05）。在这两个群体中，Proteobateria和Aeromonas在门和属水平上是最主要的细菌，并被确定为社区中的核心微生物群。通过差异丰度和KEGG途径分析发现，在门静脉感染的虾中，有两个细菌属和八个属，与肝胰腺微生物群落的改变和相关的基因功能有关。将极大丰富的Citroibacter，希瓦氏菌和暂定谱系弧菌感染的虾需要进一步调查。