Nutrient losses due to leaching from agricultural soils can be substantial but, in some cases, soil microbes such as arbuscular mycorrhizal (AM) fungi can buffer those losses. An important knowledge gap, however, is the extent to which climate change may affect AM fungal mediation of leaching via warming and drought. To investigate this, we grew lucerne (Medicago sativa) and tall fescue (Festuca arundinacea), in the presence and absence of AM fungal inoculation (Rhizophagus irregularis), under different temperature [ambient: 26 °C (aT); elevated: 30 °C (eT)] and water [well-watered: 100% soil water holding capacity (WHC); droughted: 40% WHC] treatments. After four months of plant growth, leached nutrients (PO₄^-, NH₄⁺ and NO₃^-), plant biomass, nitrogen (N), phosphorus (P), and mycorrhizal parameters (colonization and extraradical hyphae biomass) were measured. The presence of AM fungi significantly reduced P leaching with lucerne by 46% for both temperature treatments, but P loss with tall fescue was only reduced under aT (by 48%). The negation of this benefit for tall fescue under eT was associated with a reduction in mycorrhizal root colonization, while temperature did not alter mycorrhizal root colonization of lucerne. Watering regime was not observed to influence P loss via leaching. We did not find a mycorrhizal effect on N leaching for either species; however, we found that N leaching increased under drought with lucerne and responded interactively to warming and drought with tall fescue. Overall, AM fungi significantly reduced P leaching for both species, but warming eliminated this effect for tall fescue, suggesting that warming may differentially affect P leaching depending upon plant species.

由于从农业土壤中浸出造成的营养损失可能很大，但在某些情况下，土壤微生物（如丛枝菌根（AM）真菌）可以缓冲这些损失。然而，一个重要的知识鸿沟是气候变化在多大程度上可能影响AM真菌通过变暖和干旱而介导的浸出。为了研究这一点，我们长大苜蓿（紫花苜蓿）和高羊茅（高羊茅），在AM真菌接种（的存在和不存在Rhizophagus irregularis），不同的温度下[环境：26℃（AT）; 升高：30°C（eT）]和水[浇水充足：100％的土壤持水量（WHC）；干旱：40％WHC]处理。经过四个月的植物生长，营养物质浸出（PO ₄ ^-，NH₄ ⁺和NO ₃ ^-），植物生物量，氮（N），磷（P）和菌根参数（定殖和自由基菌丝生物量）被测量。在两种温度处理下，AM真菌的存在都显着地减少了使用卢塞恩的P浸出46％，但是在aT下只有高羊茅的P损失才减少了48％。在eT下对高羊茅菌的这种好处的否定与菌根根定植的减少有关，而温度并没有改变卢塞恩的菌根根定植。没有观察到浇水方式通过浸出影响磷的损失。对于这两种物种，我们均未发现氮淋失的菌根作用。然而，我们发现，在卢塞恩干旱条件下，氮的淋溶增加，而对羊茅的高热量和干旱的交互影响。总体而言，AM真菌可显着减少两种物种的P淋失，