The co-contamination of groundwater with nitrate (NO₃^--N) and manganese (Mn(II)) is a global issue that needs to be efficiently remediated. In this research, a novel denitrifying and manganese-oxidizing strain HY129 was isolated from the sediments sample of a drinking water and identified as Cupriavidus sp. HY129. The remediation ability of strain HY129 regarding the nitrate and Mn(II) pollution were investigated. The removal efficiency of nitrate and Mn(II) were 99.81% (0.229 mgL^-1h^-1) and 87.24% (0.233 mgL^-1h^-1) in bacterial culture after 72 h, respectively. Moreover, the addition of Mn(II) significantly enhanced the denitrification process, while excessive concentration of Mn(II) caused more NO₂^--N accumulation. The impacts of adsorption and oxidation activity on Mn(II) removal were investigated. Protein in extracellular polymeric substance (EPS) which produced in the Mn-oxidizing process was speculated to be the main cause of extracellular adsorption of Mn(II). Characterization of biogenic manganese oxides (BMO) confirmed the formation of high-valent manganese and the trapping experiment with sodium pyrophosphate (NaPP) demonstrated the existence of Mn(III)-intermediates. Furthermore, multicopper oxidase gene amplification provided evidence for the molecular biology of Mn(II) oxidation by strain HY129.

地下水与硝酸盐（NO ₃ ^-- N）和锰（Mn（II））的共污染是一个全球性的问题，需要进行有效的补救。在这项研究中，从饮用水的沉积物样品中分离出一种新型的反硝化和锰氧化菌株HY129，并将其鉴定为Cupriavidus sp。HY129 。研究了菌株HY129对硝酸盐和Mn（II）污染的修复能力。硝酸盐和Mn（II）的去除效率分别为99.81％（0.229 mgL ^-1 h ^-1）和87.24％（0.233 mgL ^-1 h ^-1））分别在72小时后进行细菌培养。此外，添加Mn（II）的显著增强反硝化过程，而锰（II）的浓度过高引起更多NO ₂ ^--N积累。研究了吸附和氧化活性对Mn（II）去除的影响。锰氧化过程中产生的细胞外聚合物（EPS）中的蛋白质被认为是Mn（II）细胞外吸附的主要原因。生物型锰氧化物（BMO）的表征证实了高价锰的形成，焦磷酸钠（NaPP）的捕集实验表明存在锰（III）中间体。此外，多铜氧化酶基因扩增为菌株HY129氧化Mn（II）的分子生物学提供了证据。