BACKGROUND The effect of non-surgical periodontal treatment on oral and systemic inflammatory mediators in subjects with periodontitis and hyperglycemia remains largely unknown. Therefore, the aim of this clinical study was to compare the short-term effect of non-surgical periodontal treatment on serum, saliva and GCF inflammatory markers levels in GP subjects with or without hyperglycemia. METHODS Sixty subjects divided into four groups of equal size were selected to participate: type 2 diabetics with generalized periodontitis (T2DM + GP), pre-diabetics with GP (PD + GP), normoglycemic subjects with GP (NG + GP), and healthy controls. GCF, serum, and saliva samples were obtained at baseline and 30 days after scaling and root planning (SRP) and the levels of interleukin-1β (IL-1 β), IL-8, IL-6, IL-2, IL-5, IL-4, IL-10, Interferon gamma (IFN-γ), Granulocyte macrophage colony-stimulating factor (GM-CSF) and Tumor necrosis factor-alpha (TNF-α) were determined by ultrasensitive multiplex assay. Clinical periodontal measurements were recorded. RESULTS SRP yielded significant improvement of all periodontal parameters for all GP groups (p < 0.01). A significant reduction in GCF levels of several cytokines were observed; however, only IL-1B and IFN-γ were consistently reduced post-treatment across all GP groups. Salivary levels of IL-1β were significantly reduced in all GP groups following treatment. No significant differences were observed for serum levels after SRP. CONCLUSIONS Periodontal treatment reduced local inflammatory markers, specifically IL-1B and IFN-γ, irrespective of the diabetes status. Periodontal treatment had no significant effect on serum levels of the inflammatory markers evaluated in this study.

中文翻译：

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非手术牙周治疗对局部和全身细胞因子水平的短期影响：高血糖的作用

背景在牙周炎和高血糖患者中，非手术牙周治疗对口腔和全身炎症介质的影响在很大程度上仍是未知的。因此，本临床研究的目的是比较非手术牙周治疗对伴有或不伴有高血糖的 GP 受试者血清、唾液和 GCF 炎症标志物水平的短期影响。方法 选择 60 名受试者，分为 4 组，大小相等：2 型糖尿病合并全身性牙周炎（T2DM + GP）、糖尿病前期合并 GP（PD + GP）、血糖正常的 GP（NG + GP）和健康控制。在基线和缩放和根部计划 (SRP) 后 30 天获得 GCF、血清和唾液样本以及白细胞介素 1β (IL-1 β)、IL-8、IL-6、IL-2、IL- 的水平5、IL-4、IL-10、干扰素γ（IFN-γ）、通过超灵敏多重测定法测定粒细胞巨噬细胞集落刺激因子（GM-CSF）和肿瘤坏死因子-α（TNF-α）。记录临床牙周测量。结果 SRP 显着改善了所有 GP 组的所有牙周参数（p < 0.01）。观察到几种细胞因子的 GCF 水平显着降低；然而，在所有 GP 组中，只有 IL-1B 和 IFN-γ 在治疗后持续降低。治疗后所有 GP 组的唾液 IL-1β 水平显着降低。SRP 后血清水平未观察到显着差异。结论 牙周治疗降低了局部炎症标志物，特别是 IL-1B 和 IFN-γ，与糖尿病状态无关。