Recent years have seen a vast expansion of knowledge on three-dimensional (3D) genome organization. The majority of studies on chromosome topology consists of pairwise interaction data of bulk populations of cells and therefore conceals heterogenic and more complex folding patterns. Here, we discuss novel methodologies to study the variation in genome topologies between different cells and techniques that allow analysis of complex, multi-way interactions. These technologies will aid the interpretation of genome-wide chromosome conformation data and provide strategies to further dissect the interplay between genome architecture and transcription regulation.

中文翻译：

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揭示核结构复杂性和多样性的新型正交方法

近年来，三维 (3D) 基因组组织的知识得到了广泛的扩展。大多数关于染色体拓扑的研究由大量细胞群的成对相互作用数据组成，因此隐藏了异质和更复杂的折叠模式。在这里，我们讨论了研究不同细胞之间基因组拓扑变化的新方法和允许分析复杂、多向相互作用的技术。这些技术将有助于解释全基因组染色体构象数据，并提供进一步剖析基因组结构和转录调控之间相互作用的策略。