In recent years, nanomaterials have been widely used in consumer products. High reactivity of metallic nanoparticles and its bioaccumulation in biological systems are the main causes of concern over their safety to human health and environment. The available information related to the safety of several nanomaterials is insufficient. Hematite nanoparticles are proposed for various applications. Ecotoxicological studies of hematite nanoparticles are very limited. In the present study, biosynthesised hematite nanoparticles using Bacillus cereus were evaluated for its acute oral toxicity in mice following OECD guidelines. A dose of 2 g/kg/p.o was administered to Swiss albino mice through gastric oral feeding tube and observed for 14 days. After two weeks blood samples were collected and subjected for evaluation of haematological parameters and biochemical analysis. There was no mortality and toxic signs of animals till the end of observational period. The animals were sacrificed and organs like liver and kidneys were isolated to study the histopathological changes. The results of the study revealed that there was no drastic change in parameters except slight change in bilirubin in the hematite nanoparticle treated mice. Biosynthesised hematite nanoparticles were assayed for toxicity in Artemia salina. Cysts treated with higher concentrations of hematite nanoparticles showed small sized nauplii. Biosynthesised hematite nanoparticles were found to be non-toxic to A. salina nauplii in lower concentrations.

近年来，纳米材料已广泛用于消费产品。金属纳米颗粒的高反应性及其在生物系统中的生物积累是对其对人类健康和环境安全的关注的主要原因。与几种纳米材料的安全性有关的可用信息不足。提出了赤铁矿纳米粒子用于各种应用。赤铁矿纳米粒子的生态毒理学研究非常有限。在本研究中，使用蜡状芽孢杆菌生物合成赤铁矿纳米颗粒根据OECD指南评估小鼠的急性口服毒性。通过胃口饲管向瑞士白化病小鼠施用2 g / kg / po的剂量，并观察14天。两周后，收集血液样品并进行血液学参数评估和生化分析。直到观察期结束，动物都没有死亡和毒性迹象。处死动物并分离肝和肾等器官以研究组织病理学变化。研究结果表明，在经赤铁矿纳米颗粒治疗的小鼠中，胆红素的轻微变化除外，参数没有任何大的变化。生物合成赤铁矿纳米颗粒物测定毒性卤虫。用较高浓度的赤铁矿纳米颗粒处理的囊肿显示出较小的无节幼体。发现生物合成的赤铁矿纳米颗粒在较低浓度下对盐沼无节幼体无毒。