Key message

LhGST, an anthocyanin-related GST gene, was identified from Asiatic hybrid lilies. Expression and functional analyses demonstrated that LhGST might be involved in anthocyanin sequestration in lily tepals.

Abstract

Anthocyanins are responsible for the pink, red and purple pigmentation of flowers in Asiatic hybrid lilies, synthesized at the cytoplasmic surface of the endoplasmic reticulum (ER) and then transported to the vacuole. To date, the mechanism involved in the intracellular transport of anthocyanins in lilies has not been well elucidated. Here, full-length glutathione S-transferase gene (LhGST) was identified from lilies. Expression analysis revealed that LhGST was positively correlated with anthocyanin accumulation. Phylogenetic tree analysis showed that LhGST clustered with other anthocyanin-related GSTs in the same phi clade. Moreover, functional complementation of an Arabidopsis tt19 mutant demonstrated that LhGST might be involved in anthocyanin accumulation in lily tepals. Additionally, according to phenotype analysis, LhGST was found to be correlated with the transport of anthocyanin in lilies by virus-induced gene silencing (VIGS) assay. In addition, cis-element analysis of the LhGST promoter showed the presence of ABA-, auxin-, MeJA-, gibberellin-, light-, and stress-responsive elements and an MYB recognition site (MRS, CCGTTG). Yeast one-hybrid and dual-luciferase report assays revealed that the promoter of LhGST was activated by LhMYB12-lat, which is a key R2R3-MYB transcription factor that regulates anthocyanin biosynthesis in lilies. In conclusion, our results revealed that LhGST plays a key role in anthocyanin transport and accumulation in the tepals of lilies.

中文翻译：

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LhGST 是亚洲杂交百合 (Lilium spp.) 中与花青素相关的谷胱甘肽 S-转移酶基因。

关键信息

LhGST是一种与花青素相关的GST基因，是从亚洲杂交百合中鉴定出来的。表达和功能分析表明，LhGST可能参与了百合花被片中花青素的螯合。

抽象的

花青素负责亚洲杂交百合花的粉红色、红色和紫色色素沉着，在内质网 (ER) 的细胞质表面合成，然后转运到液泡。迄今为止，百合中花青素在细胞内转运的机制尚未得到很好的阐明。在这里，从百合中鉴定出全长谷胱甘肽 S-转移酶基因 ( LhGST )。表达分析显示LhGST与花青素积累呈正相关。系统发育树分析表明，LhGST 与同一 phi 进化枝中的其他花青素相关 GST 聚类。此外，拟南芥 tt19突变体的功能互补表明LhGST可能与百合花被片中花青素的积累有关。此外，根据表型分析，通过病毒诱导的基因沉默 (VIGS) 测定发现LhGST与百合中花青素的转运有关。此外，LhGST启动子的顺式元件分析显示存在 ABA-、生长素-、MeJA-、赤霉素-、光-和应激反应元件以及 MYB 识别位点（MRS、CCGTTG）。酵母单杂交和双荧光素酶报告测定表明，LhGST的启动子被LhMYB12-lat激活，LhMYB12-lat是调节百合花青素生物合成的关键 R2R3-MYB 转录因子。总之，我们的结果表明，LhGST对花青素在百合花被膜中的转运和积累起关键作用。