Plants regularly experience multiple abiotic and biotic pressures affecting their normal development. The 90-kDa heat shock protein (HSP90) plays a dynamic role in countering abiotic and biotic stresses via a plethora of functional mechanisms. The HSP90 has been investigated in many plant species. However, there is little information available about this gene family in the cultivated Mediterranean olive tree, Olea europaea subsp. europaea var. europaea. In the current study, we systematically performed genome-wide identification and characterization of the HSP90 gene family in O. europaea var. europaea (OeHSP90s). Twelve regular OeHSP90s were identified, which were phylogenetically grouped into two major clusters and four sub-clusters, showing five paralogous gene pairs evolving under purifying selection. All of the 12 proteins contained a Histidine kinase-like ATPase (HATPase_c) domain, justifying the role played by HSP90 proteins in ATP binding and hydrolysis. The predicted 3D structure of OeHSP90 proteins provided information to understand their functions at the biochemical level. Consistent with their phylogenetic relationships, OeHSP90 members were predicted to be localized in different cellular compartments, suggesting their involvement in various subcellular processes. In consonance with their spatial organization, olive HSP90 family members were found to share similar motif arrangements and similar number of exons. We found that OeHSP90 promoters contained various cis-acting elements associated with light responsiveness, hormone signaling pathways and reaction to various stress conditions. In addition, expression sequence tags (ESTs) analysis offered a view of OeHSP90 tissue- and developmental stage specific pattern of expression. Proteins interacting with OeHSP90s were predicted and their potential roles were discussed. Overall, our results offer premises for further investigation of the implication of HSP90 genes in the physiological processes of the olive and its adaptation to stresses.

植物经常受到影响其正常发育的多种非生物和生物压力。90-kDa 热休克蛋白 (HSP90) 通过多种功能机制在对抗非生物和生物胁迫方面发挥着动态作用。已经在许多植物物种中研究了 HSP90。然而，在栽培的地中海橄榄树Olea europaea subsp. 中，关于该基因家族的信息很少。欧洲变种 欧洲。在目前的研究中，我们系统地对O. europaea var.中的 HSP90 基因家族进行了全基因组鉴定和表征。欧洲（OeHSP90s）。确定了十二个常规 OeHSP90，它们在系统发育上分为两个主要簇和四个子簇，显示了五个旁系同源基因对在纯化选择下进化。所有 12 种蛋白质都含有组氨酸激酶样 ATP 酶 (HATPase_c) 结构域，证明了 HSP90 蛋白质在 ATP 结合和水解中所起的作用。OeHSP90 蛋白质的预测 3D 结构提供了在生化水平上了解其功能的信息。与它们的系统发育关系一致，OeHSP90 成员被预测定位于不同的细胞区室，表明它们参与了各种亚细胞过程。与其空间组织一致，橄榄 HSP90 家族成员被发现具有相似的基序排列和相似数量的外显子。我们发现 OeHSP90 启动子包含与光反应性、激素信号通路和对各种压力条件的反应相关的各种顺式作用元件。此外，表达序列标签 (EST) 分析提供了 OeHSP90 组织和发育阶段特定表达模式的视图。预测了与 OeHSP90 相互作用的蛋白质，并讨论了它们的潜在作用。总的来说，我们的结果为进一步调查的影响提供了前提 预测了与 OeHSP90 相互作用的蛋白质，并讨论了它们的潜在作用。总的来说，我们的结果为进一步调查的影响提供了前提 预测了与 OeHSP90 相互作用的蛋白质，并讨论了它们的潜在作用。总的来说，我们的结果为进一步调查的影响提供了前提橄榄生理过程中的HSP90基因及其对压力的适应。