The use of nanomaterials as a matrix for soft ionization mass spectrometry has been investigated and reported various times. Under certain conditions, measurements of samples containing small molecules enhanced by nanomaterials have better sensitivity compared with other methods. Nanoscaled Ag prepared by various methods was reported as a substrate for surface-assisted LDI MS. Higher desorption and (in some cases) charge transfer through adduct formation with silver ion was reported. But primary ionization mechanism was through attachment of proton or alkali ion. Our approach is to overcoat the sample by Ag nanoparticles (the so-called nanoparticle-assisted LDI MS or nanoPALDI MS). As nanoparticles having adsorption maxima close to the laser wavelength, we expect an improvement in desorption enhancement, release of silver ions and (as a result of latter) increased adduct formation of [analyte+Ag]⁺ ions. The nanoparticles are produced by gas aggregation under low-pressure, making the process homogeneous over relatively large areas, free from impurities, and absence of capping agent often presented in colloidal nanoparticles. This work reports the detection of natural amino acids with Ag nanoparticles as a matrix. We successfully detected all amino acids (as [analyte+Ag]⁺ ion), including in the mixtures of few amino acids. Moreover, a formation of other adducts, particularly [analyte+Ag₃]⁺ and [analyte₂+Ag]⁺ is reported. The differences in ion intensity ratios depending on amino acid types are discussed. The obtained results showed that laser ablation is more efficient for amino acids with hydrophobic, unique, and neutral side chains.

纳米材料作为软电离质谱法的基质已被研究和报道过多次。在某些条件下，与其他方法相比，使用纳米材料增强的包含小分子的样品的测量具有更好的灵敏度。据报道，通过各种方法制备的纳米级Ag作为表面辅助LDI MS的底物。据报道，通过与银离子形成加合物，可以实现更高的解吸和（在某些情况下）电荷转移。但是主要的电离机理是通过质子或碱离子的附着。我们的方法是通过Ag纳米颗粒（所谓的纳米颗粒辅助LDI MS或nanoPALDI MS）对样品进行覆盖。由于纳米粒子的吸附最大值接近激光波长，因此我们期望其解吸增强作用得到改善，[分析物+ Ag] ⁺离子。纳米颗粒是通过低压下的气体聚集而产生的，从而使该过程在相对较大的区域内均匀，没有杂质，并且胶体纳米颗粒中通常不存在封端剂。这项工作报告了以Ag纳米颗粒为基质对天然氨基酸的检测。我们成功地检测了所有氨基酸（作为[分析物+ Ag] ⁺离子），包括少数氨基酸的混合物中。此外，形成其他加合物，特别是[分析物+ Ag ₃ ] ⁺和[分析物₂ + Ag] ⁺被报道。讨论了取决于氨基酸类型的离子强度比的差异。获得的结果表明，激光消融对具有疏水，独特和中性侧链的氨基酸更有效。