Cancer patients who have a diagnosis in the early stage of the disease have better chances for therapy. Thus, development of new diagnostic procedures is needed for the early detection of cancer. Measuring and analyzing the light that is scattered by the cells can be utilized to monitor early variations associated with cancer evolution. The key hypothesis behind this research was the application of the Z-scan method to progress the precision of detecting the C6 glioblastoma multiforme cancer cells and the OLN-93 normal glial cells.

A Nd:YAG CW laser with a wavelength of 532 nm was used. The laser beam passed through the prepared samples and the transmittance power is distinguished by dislocation. Finally, the nonlinear refractive index and the extinction coefficient of the samples were determined.

Our study showed that the extinction coefficient of the C6 and OLN-93 cells were 49 3 and 33 2, respectively. Also, the sign and value of the nonlinear refractive index (n₂) for the C6 and OLN-93 cells was −5.44 10⁻⁷ cm² w⁻¹ and +6.07 10⁻⁷ cm² w⁻¹, respectively. According to this study, the sign of the n₂ index for the C6 and OLN-93 cells was negative and positive, respectively.

Our results suggested that the nonlinear refractive index of the cell samples and Z-scan technique could be an applicable means for identifying glial cancer cells from normal cells.

在疾病早期得到诊断的癌症患者有更好的治疗机会。因此，需要开发新的诊断程序来早期检测癌症。测量和分析细胞散射的光可用于监测与癌症发展相关的早期变异。这项研究背后的关键假设是应用Z扫描法提高检测C6胶质母细胞瘤多形性癌细胞和OLN-93正常神经胶质细胞的准确性。

使用波长为532 nm的Nd：YAG CW激光器。穿过制备的样品的激光束和透射功率通过位错来区分。最后，确定了样品的非线性折射率和消光系数。

我们的研究表明，C6和OLN-93细胞的消光系数分别为49 3和33 2。另外，C6和OLN-93单元的非线性折射率（n ₂）的符号和值分别为-5.44 10 ^-7 cm ² w ^-1和+6.07 10 ^-7 cm ² w ^-1。根据这项研究，C6和OLN-93细胞的n ₂指数分别为负和正。

我们的结果表明，细胞样品的非线性折射率和Z扫描技术可能是从正常细胞中鉴定神经胶质癌细胞的一种适用方法。