ABSTRACT

Several plant phytochemicals have been reported to possess therapeutic properties. However, most of them are capable of causing oxidation of the human haemoglobin into the ferric state making them hazardous to cells and tissues. This study was aimed at investigating the non-oxidative effect of the flavonoid-rich fraction of Lasianthera africana leaves (LAFRF) on human haemoglobin. Isolation and purification of LAFRF was carried out by solvent extraction, partitioning and ion-exchange chromatography. Six test groups were setup as follows: A reaction tube containing 800 µl of oxyhaemoglobin (OxyHb) served as the normal control, while the test groups were made up of different concentrations of LAFRF (5, 10, 20, 40 and 80 mg/ml) incubated with 100 µl of OxyHb. The non-oxidative effects of the LAFRF on the human haemoglobin was assessed by UV-visible spectroscopy. The results indicated that LAFRF possess non-oxidative effects on human haemoglobin, as it did not cause any disappearance of the absorbance maxima at 576 nm and 541 nm at all concentrations investigated. Furthermore, a concentration-dependent increase in the absorbance of the treated OxyHb was recorded when compared to the untreated OxyHb, thus signifying its potential as a safe pharmacological agent for in vivo investigations.

摘要

据报道几种植物植物化学物质具有治疗特性。然而，它们中的大多数能够引起人血红蛋白氧化成三价铁态，从而对细胞和组织有害。这项研究的目的是调查非洲菊中富含类黄酮的部分的非氧化作用在人血红蛋白上留下（LAFRF）。通过溶剂萃取，分配和离子交换色谱法进行LAFRF的分离和纯化。设置六个测试组，如下所示：包含800 µl氧合血红蛋白（OxyHb）的反应管用作正常对照组，而测试组则由不同浓度的LAFRF（5、10、20、40和80 mg / ml）组成。 ）与100 µl OxyHb孵育。LAFRF对人血红蛋白的非氧化作用通过紫外可见光谱法评估。结果表明，LAFRF对人血红蛋白具有非氧化作用，因为在所有所研究的浓度下，LAFRF均不会导致最大吸光度在576 nm和541 nm处消失。此外，体内调查。