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Biodegradation of cyanide in cassava wastewater using a novel thermodynamically-stable immobilized rhodanese
Preparative Biochemistry & Biotechnology ( IF 2.9 ) Pub Date : 2020-11-18 , DOI: 10.1080/10826068.2020.1846053
Adedeji Nelson Ademakinwa 1 , Mayowa Oladele Agunbiade 2 , Oladapo Fagbohun 3
Affiliation  

Abstract

Extracellular rhodanese obtained from Aureobasidium pullulans was employed in both free and immobilized forms for the biodegradation of cyanide present in cassava processing mill effluent (CPME). Crosslinking with glutaraldehyde (at an optimum concentration of 5% v/v) before entrapment in alginate beads resulted in the highest immobilization yield of 94.5% and reduced enzyme leakage of 1.8%. Rhodanese immobilized by cross-linking before entrapment (cbe) retained about 46% of its initial activity after eight cycles of catalysis compared to the entrapment in alginate alone (eaa) which lost more than 79% after the fifth catalytic cycle. A cross-examination of thermodynamic (ΔGd*, ΔSd*, ΔHd*) kinetic (kd, t1/2, D and zvalues) parameters at 30–70 °C showed that cbe displayed a higher resistance to thermal inactivation when compared to the free enzyme (fe) and (eaa). The efficiency of cyanide biodegradation from the CPME by the fe, eaa and cbe were 55, 62, and 74% respectively after 6 h. Rhodanese immobilized via cbe had a higher resistance to thermal denaturation over other enzyme forms. Hence, this makes cbe adaptable for large-scale detoxification of cyanide from CPME.



中文翻译:

使用新型热力学稳定的固定化硫氰酸盐生物降解木薯废水中的氰化物

摘要

Aureobasidium pullulans获得的细胞外硫氰酸酶以游离和固定形式用于生物降解木薯加工工厂废水 ( CPME ) 中存在的氰化物。用戊二醛(以 5% v/v 的最佳浓度)在包埋在藻酸盐珠中之前交联导致了 94.5% 的最高固定产率和 1.8% 的酶泄漏减少。与单独的海藻酸盐 ( eaa )捕获相比,捕获前通过交联固定的硫氰酸( cbe)在八次催化循环后保留了约 46% 的初始活性,相比之下,在第五次催化循环后损失超过 79%。热力学交叉检查(ΔGd*, Δd*, ΔHd*) 动力学 (d, 1/2, D  z-价值观) 30–70 °C 的参数表明,与游离酶 ( fe ) 和 ( eaa )相比,cbe显示出更高的热灭活抵抗力。氰化物生物降解从效率CPMEFE,EAACBE 6小时后分别为55,62和74%。与其他酶形式相比,通过cbe固定的硫氰酸酶对热变性具有更高的抵抗力。因此,这使得cbe适用于CPME中氰化物的大规模解毒

更新日期:2020-11-18
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