Graphene has attracted great interest for its uses in tissue regeneration. Multilayered graphene hydrogel (MGH) membranes show great potential as barrier membranes in guided bone regeneration. However, when using the conventional methyl methacrylate‐embedding process, the MGH membrane swells and loses its natural form, which seriously limits the observation of bone regeneration. A rapid embedding method is developed by adding the cross‐linker tetra(ethylene glycol)diacrylate and increasing the polymerization temperature (45 °C) and initiator concentration (64% benzoyl peroxide in penetration liquid). By the rapid embedding method, the shape and properties of the MGH membrane can be well preserved, and the bone tissue can also be well embedded. The resulting blocks have uniform hardness, which makes it possible to obtain thin sections using a cutting and grinding procedure. The rapid embedding method has no significant effect on the conventional methods of bone regeneration detection. The interface between the MGH membrane and new bone tissue can be clearly observed. This new hard‐tissue‐embedding method can lead to better observation of the interaction between bone and graphene, providing an optimized experimental technology for the study of hard tissue regeneration with graphene in the future.

中文翻译：

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嵌入石墨烯纳米材料的快速硬组织嵌入方法：多层石墨烯水凝胶膜

石墨烯因其在组织再生中的用途而引起了极大的兴趣。多层石墨烯水凝胶（MGH）膜在引导性骨再生中显示出作为屏障膜的巨大潜力。但是，当使用常规的甲基丙烯酸甲酯包埋工艺时，MGH膜会膨胀并失去其天然形式，这严重限制了对骨再生的观察。通过添加交联剂四（乙二醇）二丙烯酸酯并提高聚合温度（45°C）和引发剂浓度（渗透液中64％的过氧化苯甲酰），开发了一种快速包埋方法。通过快速包埋法，可以很好地保持MGH膜的形状和特性，并且可以很好地嵌入骨组织。产生的块具有均匀的硬度，这样就可以使用切割和磨削程序获得薄的切片。快速嵌入方法对常规的骨再生检测方法没有显着影响。可以清楚地观察到MGH膜和新骨组织之间的界面。这种新的硬组织嵌入方法可以更好地观察骨骼与石墨烯之间的相互作用，为将来研究石墨烯硬组织再生提供了一种优化的实验技术。