Raman spectroscopy, an established optical method of remote diagnostics of pigments and dyes, is applied here for the first time to the cup fungus Scarlet elf cup (Sarcoscypha coccinea or Plectania coccinea or Peziza coccinea) from the order Pezizales (Ascomycota). As‐collected fungi samples are investigated, not subject to any treatment. The dominant vibrational features in the acquired in vivo spectrum, around 1000, 1150, and 1500 cm⁻¹, respectively, clearly indicate it is dominated by carotenoid(s). Contrary to most previous Raman studies of carotenoids, including few studies of fungi, which focused on the latter three strongest fundamental vibrations, we have resolved and analyzed in detail 30 vibrational bands, including rarely studied wavenumber range above 2000 cm⁻¹. We discuss in detail the inferred from our spectral analysis carotenoid species in comparison with those previously reported for Sarcoscypha coccinea by other methods. In particular, we suggest two characteristic ring vibrations, at 1131 and 1283 cm⁻¹, to be used as a measure of the ratio of astaxanthin and plectaniaxanthin to β‐carotene and lycopene in this and similar fungi. In addition, we report the fluorescence spectrum of this fungal species, present as background in the Raman spectrum at visible excitation. The results obtained in this work will facilitate subsequent more quantitative studies of carotenoid composition in other kinds of fungi and other microorganisms using Raman spectroscopy in combination with other techniques.

中文翻译：

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用拉曼光谱法分析猩红精灵杯（Sarcoscypha coccinea）类胡萝卜素的体内作用

拉曼光谱法是一种建立了的远程诊断颜料和染料的光学方法，在此首次将其用于杯状真菌猩红色小精灵杯（Sarcoscypha coccinea或Plectania coccinea或Peziza coccinea），顺序为Pezizales（亚目）。对收集的真菌样品进行了调查，未进行任何处理。在所获得的体内光谱中，大约1000、1150和1500 cm ^-1的主要振动特征分别清楚地表明它以类胡萝卜素为主。与以往的大多数对类胡萝卜素的拉曼研究（包括针对后三个最强基本振动的真菌研究）相反，我们已经详细解析并分析了30个振动带，其中包括很少研究的2000 cm ^-1以上的波数范围。我们将详细讨论从光谱分析类胡萝卜素种类推论得出的结果，并与先前通过其他方法报道的关于球藻的结果进行比较。特别是，我们建议在1131和1283 cm ^-1处出现两个特征性的环振动。，用于测量该真菌和类似真菌中虾青素和lectantaniaxanthin与β-胡萝卜素和番茄红素的比例。此外，我们报告了这种真菌物种的荧光光谱，在可见光激发下作为背景出现在拉曼光谱中。这项工作中获得的结果将有助于随后使用拉曼光谱法结合其他技术对其他种类的真菌和其他微生物中的类胡萝卜素成分进行更定量的研究。